Polynucleotide and polypeptide sequences encoding rat mdr1a and screening methods thereof
OwnerKimberly Anne Brun, Richard James Chenery +3inventors
InventorsKimberly Anne Brun, Richard James Chenery, Harma Ellens, John Anthony Feild, Lin Yue
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Abstract
Rat mdr1a polypeptides and polynucleotides and method for producing such polypeptides by recombinant techniques are disclosed. Also disclosed are methods for screening for compounds which either agonize or antagonize rat mdr1a. Further disclosed is method for performing a selection screen, whereby compounds are discovered that neither agonize nor antagonize rat mdr1a.
Claims12
An isolated polypeptide selected from the group consisting of:
- (i) an isolated polypeptide having at least a 95% identity to the amino acid sequence of SEQ ID NO:2 over the entire length of SEQ ID NO:2;
- (ii) an isolated polypeptide comprising the amino acid sequence of SEQ ID NO:2 or
- (iii) an isolated polypeptide which is the amino acid sequence of SEQ ID NO:2.
An isolated polynucleotide selected from the group consisting of:
- (i) an isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide that has at least a 95% identity to the amino acid sequence of SEQ ID NO:2, over the entire length of SEQ ID NO:2;
- (ii) an isolated polynucleotide comprising a nucleotide sequence that has at least a 95% identity over its entire length to a nucleotide sequence encoding the polypeptide of SEQ ID NO:
- 2;
- (iii) an isolated polynucleotide comprising a nucleotide sequence which has at least a 95% identity to that of SEQ ID NO:1 over the entire length of SEQ ID NO:1;
- (iv) an isolated polynucleotide comprising a nucleotide sequence encoding the polypeptide of SEQ ID NO:2;
- (vi) an isolated polynucleotide which is the polynucleotide of SEQ ID NO:1;
- or
- (vi) an isolated polynucleotide obtainable by screening an appropriate library under stringent hybridization conditions with a labeled probe having the sequence of SEQ ID NO:1 or a fragment thereof.;
- or a nucleotide sequence complementary to said isolated polynucleotide.
A process for producing a recombinant host cell comprising transforming or transfecting a cell with the expression system of claim 8 such the the host cell, under appropriate culture conditions, produces a polypeptide comprising an amino acid sequence having at least a 95% identity to the amino acid sequence of SEQ ID NO:2 over the entire length of SEQ ID NO:2.
An isolated polynucleotide selected form the group consisting of:
- (a) an isolated polynucleotide comprising a nucleotide sequence which has at least 95% identity to SEQ ID NO:3 over the entire length of SEQ ID NO:3;
- (b) an isolated polynucleotide comprising a nucleotide sequence which has at least 95% identity to SEQ ID NO:1
- over the entire length of SEQ ID NO:3;
- (e) an isolated polynucleotide comprising the polynucleotide of SEQ ID NO:3;
- (d) the polynucleotide of SEQ ID NO:3;
- or
- (e) an isolated polynucleotide comprising a nucleotide sequence encoding a polypeptide which has at least 95% identity to the amino acid sequence of SEQ ID NO:4, over the entire length of SEQ ID NO:
- 4.
A polypeptide selected from the group consisting of:
- (a) a polypeptide which comprises an amino acid sequence which has at least 95% identity to that of SEQ ID NO:4 over the entire length of SEQ ID NO:4;
- (b) a polypeptide in which the amino acid sequence has at least 95% identity to the amino acid sequence of SEQ ID NO:4 over the entire length of SEQ ID NO:4;
- (c) a polypeptide which comprises the amino acid of SEQ ID NO:4;
- (d) a polypeptide which is the polypeptide of SEQ ID NO:4;
- (e) a polypeptide which is encoded by a polynucleotide comprising the sequence contained in SEQ ID NO:3.
Dependencies are inferred from the claim text.