Thermostable alkaline phosphatases

A purified enzymatically active portion of the thermostable alkaline phosphatase present in Rhodothermus marinus having an apparent subunit molecular weight of about 45,000-55,000 as determined by SDS-PAGE and stable to heating at 65° C. for 1 hour.

Recombinant alkaline phosphatase derived from Rhodothermus marinus having an apparent subunit molecular weight of about 45,000-55,000 as determined by SDS-PAGE gel and stable to heating at 65° C. for 1 hour.

Method for detecting nucleic acid or protein in sample comprising the step of providing a nucleic acid, protein, or antibody labelled with a thermostable alkaline phosphatase which is stable to heating at 65° C. for 1 hour.

Method for detecting nucleic acid or protein in sample comprising the step of providing a nucleic acid, protein, or antibody labelled with a thermostable alkaline phosphatase derived from Rhodothermus marinus which is stable to heating at 65° C. for 1 hour.

Recombinant DNA encoding a thermostable alkaline phosphatase present in Thermus thermophilus having a subunit size of about 51,000 Daltons and retaining at least 10% of its alkaline phosphatase activity after heating at 65° C. for one hour.

Recombinant alkaline phosphatase corresponding to that present in Thermus thermophilus having a pH optimum greater than 10.5 and stable to heating at 65° C. for 1 hour.

Method for detecting nucleic acid or protein in sample comprising the step of providing a nucleic acid, protein, or antibody labelled with a thermostable alkaline phosphatase from Thermus thermophilus having a pH optimum greater than 10.5 and stable to heating at 65° C. for 1 hour.

Recombinant DNA encoding a thermostable alkaline phosphatase obtainable by amplification using an oligonucleotide probe corresponding to the amino terminal sequence from Thermus thermophilus as a 5′ end primer with total genomic DNA from Thermus thermophilus as a template.

A purified enzymatically active portion of the thermostable alkaline phosphatase present in Thermus thermophilus having a pH optimum greater than 10.5 and stable to heating at 65° C. for 1 hour.

A purified enzymatically active portion of the thermostable alkaline phosphatase present in Thermosipho africanus having an apparent molecular weight of about 47,000 Daltons in a 4-15% SDS polyacrylamide gel and stable to heating at 65° C. for 1 hour.

Recombinant DNA encoding a thermostable alkaline phosphatase present in Thermosipho africanus having an apparent molecular weight of about 47,000 Daltons in a 4-15% SDS polyacrylamide gel and stable to heating at 65° C. for 1 hour.

Recombinant alkaline phosphatase present in Thermosipho africanus having an apparent molecular weight of about 47,000 Daltons in a 4-15% SDS polyacrylamide gel and stable to heating at 65° C. for 1 hour.

Method for detecting nucleic acid or protein in sample comprising the step of providing a nucleic acid, protein, or antibody labelled with a thermostable alkaline phosphatase present in Thermosipho africanus having a pH optimum greater than 10.5 and stable to heating at 65° C. for 1 hour.