Method of detection of influenza virus and compounds for use therein
OwnerPhillip A. Reece, Wen-Yang Wu +3inventors
InventorsPhillip A. Reece, Wen-Yang Wu, Betty Jin, Guy Y. Krippner, Keith Geoffrey Watson
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Abstract
The invention provides a method of detection of influenza virus which utilises compounds able to bind specifically to the active site of influenza virus neuraminidase, and novel compounds for use in the method.
Claims41
A method of detection of influenza virus, comprising the step of exposing a sample suspected to comprise said virus to a compound (neuraminidase binder) able to bind specifically to the active site of influenza virus neuraminidase.
A method according to any one of the preceding claims, in which the neuraminidase binder has an IC50 for binding of less than 10−6 M.
A neuraminidase binder comprsiing an analogue of neuraminic acid (sialic acid) substituted at the C7 or equivalent position with a spacer group suitable for coupling to a detectable label or to a surface, in which said analogue does not comprise a detectable label or spacer group which is cleaved by the neuraminidase.
5-acetamido-7-(6′-biotinylamino-triglycinamido-hexyl) -carbamoyloxy-4-guanidino-2,3,4,5-tetradeoxy-D-glycero-D-galacto-non-2-enopyranosonic acid (21).
5-acetamido-7-(6′-(6″-(6′″-aminocaproyl) -triaminocaproyl)-aminohexyl)-carbamoyloxy-4-guanidino -2,3,4,5-tetradeoxy-D-glycero-D-galacto-non-2-enopyranosonic acid (25).
5-acetamido-7-{6′-[6″-(6′″-biotinylaminocaproyl) -tri-aminocaproyl]-aminohexyl}-carbamoyloxy-4-guanidino -2,3,4,5-tetradeoxy-D-glycero-D-galacto-non-2-enopyranosonic acid (26).
5-acetamido 7-{6′-[6″-(6′″-hydrazidosuccinylaminocaproyl)-triaginocaproyl]-aminohexyl}-carbamoyloxy-4-guanidino-2,3,4,5-tetradeoxy-D-glycero-D-galacto-non-2-enopyranosonic acid (30).
Dependencies are inferred from the claim text.