Determination of metal ions in solution by photoluminescence anisotropy

A kit for assay of divalent metal ion concentration in a sample comprising:

• i) an apo-carbonic anhydrase protein conjugated to a photoluminescent molecule selected from the group consisting of dansyl aziridine, 4-chloro-7-sulfobenzofuran, 7-flurorbenz-2-oxa-1,3-diazole-4-sulfonamide and nitrobenzoxadiazolyl;
• ii) optionally a standard solution of at least one divalent metal ion;
• iii) optionally a buffer for maintaining a concentration of free divalent metal ions in a solution;
• and
• iv) optionally a chelating resin to prevent or remove unwanted metal contamination;
• said items i), ii), iii) and iv) being packaged in a container that prevents unwanted contamination by divalent metal ions.

A kit for assay of divalent metal ion concentration in a sample comprising:

• i) an apo-carbonic anhydrase protein;
• ii) a photoluminsecent molecule selected from the group consisting of 4-aminosulfonyl[1-(4-N-(5-fluoresceinylthioureido)butyl]benzamide, 7-flurorbenz-2-oxa-1,3-diazole-4-sulfonamide:
• p-mercaptoethanol adduct, dansylamide, hydroxynaphthalenesulphonamide, 2- (3-methoxy-4-ethoxyphenyl)-4-chloroquinoline-6-sulfonamide, N-(1-anthracenyl)-4-sulfonamido-benzenesulfonamide, ethyl-2-(4-sulfonamidophenyl)-4-hydroxyquinoline-6-carboxylate and N-(N′-(4′-sulfamoylglutaranily-amidoethyl))-4-amino-3,6-disulfo-1,8-naphthalimide
• iii) optionally a standard solution of a divalent metal ion;
• iv) optionally a buffer for maintaining a concentration of free divalent metal ion in a solution;
• and
• v) optionally a chelating resin to prevent or remove unwanted metal contamination;
• said items i), ii), iii), iv) and v) being packaged in a container that prevents unwanted contamination by divalent metal ions.