Composition for density gradient cell separation
OwnerSteven M. Woodsideinventor
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Abstract
A novel composition for density gradient separation is disclosed. The composition comprises (a) a red blood cell aggregating agent and (b) a density enhancing agent wherein the composition is iso-osmolar to the sample it is used on. Preferably, the osmolarity of the composition is from about 270 to about 300 mOsm and the red blood cell aggregating agent is hetastarch.
Claims16
A composition for the isolation of nucleated cells from a sample by density gradient separation comprising (a) a red blood cell aggregating agent;
- and (b) a density enhancing agent wherein the composition is iso-osmolar to the sample.
A method for the isolation of nucleated cells from a sample by density gradient separation comprising (1) layering the sample over one or more layers of different density of a composition comprising (a) a red blood cell aggregating agent and (b) a density enhancing agent wherein the composition is iso-osmolar to the sample (2) centrifuging the sample that is layered over the composition;
- and (3) isolating the nucleated cells from the composition:plasma interface.
Dependencies are inferred from the claim text.