Methods and compositions for bone graft implants
OwnerGlenn Morris Gainey, Regina Landesberginventors
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Abstract
An autologous platelet gel for bone grafts is comprised of a mixture of platelet-rich-plasma activated by calcium chloride solution, and an aqueous suspension of partially frayed Type I collagen.
Claims12
An autologous gel, comprising;
- an aqueous suspension of Type I fibrillar collagen alkaline treated to created partially frayed termini;
- calcium chloride;
- and platelet-rich-plasma processed to form an in vitro formable matrix.
A method of making an implant gel, comprising the steps of:
- making (( an admixture of Type I collagen in physiological suspension, an aqueous ( solution of calcium chloride and bone particulate;
- and adding to said admixture platelet-rich-plasma in an amount sufficient to establish a viscous formable gel.
In a platelet-based sealant characterized by a mixture of calcium chloride, thrombin and a source of platelets, the improvement comprising:
- substituting an effective amount of terminally frayed Type I microfibrillar collagen for said thrombin.
A cellular matrix for cellular moieties, comprising by volume 10 to 60 parts of platelet-rich-plasma;
- 1 to 5 parts calcium chloride;
- 5 to 60 parts of partially defibrillated Type I collagen;
- and 5 to 40 parts bone particulate.
A method of making an in-vitro thrombic agent for use in platelet-rich-plasma compositions comprising the steps of:
- forming a mixture of Type I fibrillar collagen and water;
- adjusting the mixture to a pH in the range of about 8 to 12;
- stirring said mixture at said range for a sufficient time to establish a stable suspension;
- and readjusting the suspension to a pH in the physiological range.
The method as recited in claim 7 wherein said mixture has a pH in the range of 9.5 to 110 and said physiological range is between 6.8 and 7.2.
A method of separating from whole blood a predominantly platelet-rich-plasma fraction comprising the steps of:
- centrifuging a volume of whole blood at a first separating condition at centrifugal forces in the range of about 150 g to 400 g and a time sufficient to differentiate a top layer, a middle layer and a bottom layer;
- separating said top layer and said middle layer from said bottom layer;
- recentrifuging the top layer and the middle layer at a second separating condition at centrifugal forces in the range of about 225 g to 300 g for a time to differentiate further said top layer from said middle layer;
- and separating said middle layer from said top layer.
The method as recited in claim 9 wherein said centrifugal forces in at said first separating condition are in the range of about 175 g to 250 g.
A cellular matrix comprising:
- a cellular moiety in admixture with an autologous platelet-based gel including autologous platelet-rich-plasma, terminally frayed microfibrillar Type I collagen, and a gelling initiator.
Dependencies are inferred from the claim text.