Bacterial strain, processed plant extracts, compositions containing same, processes for their preparation and their therapeutic and industrial applications

A probiotic composition comprising viable Escherichia coli strain BU-230-98 ATCC Deposit No. 20226 (DSM 12799) and a formulation for maintaining viability of said Escherichia coli strain.

A formulation comprising at least one volatile fraction (VF) of a plant extract, said volatile fraction being prepared by steam distillation of said plant extract under reduced pressure and at a bath temperature not exceeding 38° C.

A veterinary feed additive for enhancing animal weight gain comprising at least one volatile fraction (VF) of a plant extract, said volatile fraction being prepared by steam distillation of said plant extract under reduced pressure and at a bath temperature not exceeding 38° C.

A food additive for enhancing weight gain in a recipient thereof, the food additive comprising at least one volatile fraction (VF) of a plant extract, said volatile fraction being prepared by steam distillation of said plant extract under reduced pressure and at a bath temperature not exceeding 38° C.

A probiotic composition comprising:

• (a) an effective amount of at least one viable probiotic microorganism having a beneficial biological or therapeutic activity in the gastrointestinal tract;
• and
• (b) at least one volatile fraction (VF) of a plant extract.

A composition comprising:

• (a) at least one microorganism;
• and
• (b) at least one volatile fraction (VF) of a plant extract, said at least one volatile fraction is selected so as to sustain viability of said at least one microorganism for at least 2 months at room temperature.

A process of preparing a therapeutic formulation, comprising the step of mixing at least one volatile fraction (VF) of a plant extract with physiologically or veterinary acceptable additives, carriers or diluents.

A process of growing Escherichia coli strain BU-230-98 ATCC Deposit No. 20226 (DSM 12799), the process comprising the step of seeding a starter cloture of Escherichia coli strain BU-230-98 ATCC Deposit No. 20226 (DSM 12799) into a growth media and growing said Escherichia coli strain up to optical density of 15-30 at 650 nm.

A process of manufacturing a probiotic composition, the process comprising the step of suspending viable Escherichia coli strain BU-230-98 ATCC Deposit No. 20226 (DSM 12799) in a formulation for maintaining viability of said Escherichia coli strain.

A process of preparing a probiotic composition, the process comprising the step of suspending an effective amount of at least one viable probiotic microorganism having a beneficial biological or therapeutic activity in the gastrointestinal tract in an formulation containing at least one volatile fraction (VF) of a plant extract.

A process of preparing a composition including viable microorganisms, the process comprising the step of suspending at least one microorganism in a formulation containing at least one volatile fraction (VF) of a plant extract, said at least one volatile fraction is selected so as to sustain viability of said at least one microorganism for at least 2 months at room temperature.

A dispenser of microorganisms comprising a reservoir and a dispensing mechanism being connected thereto, said reservoir housing at least one microorganism in a formulation containing at least one volatile fraction (VF) of a plant extract, said at least one volatile fraction is selected so as to sustain viability of said at least one microorganism for at least 2 months at room temperature.

A method of biocontrol of an organism, the method comprising the step of spreading in an endemic area at least one viable microorganism capable of biocontrolling the organism, said at least one viable microorganism being in a formulation containing at least one volatile fraction (VF) of a plant extract, said at least one volatile fraction is selected so as to sustain viability of said at least one microorganism for at least 2 months at room temperature.

A method of eliminating an oil pollution, the method comprising the step of spreading in a polluted area at least one viable microorganism capable of degrading oil, said at least one viable microorganism being in a formulation containing at least one volatile fraction (VF) of a plant extract, said at least one volatile fraction is selected so as to sustain viability of said at least one microorganism for at least 2 months at room temperature.

A method of restoring organic fumes degrading microorganism population in a biofilter, the method comprising the step of dispensing onto the filter at least one viable microorganism capable of degrading organic fumes, said at least one viable microorganism being in a formulation containing at least one volatile fraction (VF) of a plant extract, said at least one volatile fraction is selected so as to sustain viability of said at least one microorganism for at least 2 months at room temperature.

A food product supplemented with viable Escherichia coli strain BU-230-98 ATCC Deposit No. 20226 (DSM 12799).

A method of preparing a starter for a fermentation process, the method comprising the step of growing a sufficient amount of a starter microorganism and suspending said starter microorganism in a formulation containing at least one volatile fraction (VF) of a plant extract, said at least one volatile fraction is selected so as to sustain viability of said starter microorganism for at least 2 months at room temperature.