Anaerobic bacterium as a drug for cancer gene therapy
OwnerMinoru Fujimori, Shun?apos;ichiro Taniguchi +5inventors
InventorsMinoru Fujimori, Shun?apos;ichiro Taniguchi, Jun Amano, Kazuyuki Yazawa, Yasunobu Kano, Toshiyuki Nakamura, Takayuki Sasaki
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Abstract
The present invention provides a bacterium belonging to the genus Bifidobacterium, by which DNA coding for a protein having an antitumor activity or DNA coding for a protein having the activity of converting a precursor of an antitumor substance into the antitumor substance is delivered to tumor tissues specifically under anaerobic conditions thereby expressing the protein encoded by the DNA, as well as a pharmaceutical composition comprising said anaerobic bacterium.
Claims27
A method for delivering a gene in a system for delivering DNA specifically to tumor tissues under anaerobic conditions, wherein a bacterium belonging to the genus Bifidobacterium is used as a gene delivery vector and then the DNA delivered specifically to tumor tissues under anaerobic conditions is expressed in said tumor tissues.
A method for delivering a gene in a system for delivering DNA specifically to tumor tissues under anaerobic conditions, wherein a bacterium belonging to the genus Bifidobacterium and having the DNA coding for a protein which has a higher activity than in its parent strain is used as a gene delivery vector and then the DNA delivered specifically to tumor tissues under anaerobic conditions is expressed in said tumor tissues.
A method for delivering a gene in a system for delivering DNA specifically to tumor tissues under anaerobic conditions, wherein a bacterium belonging to the genus Bifidobacterium transformed with a recombinant DNA having said DNA is used as a gene delivery vector and the DNA delivered specifically to tumor tissues under anaerobic conditions is expressed in the tumor tissues.
Bifidobacterium longum 105-A/pBLES100-S-eCD (FERM BP-7274.
DNA having the nucleotide sequence set forth in SEQ ID NO:
- 1.
An anaerobic bacterium belonging to the genus Bifidobacterium capable of expressing a gene coding for a protein having an antitumor activity in only cancer cells under substantially anaerobic conditions.
An anaerobic bacterium belonging to the genus Bifidobacterium capable of expressing a gene coding for a protein having the activity of converting a precursor of an antitumor substance with low toxicity to humans and animals into an antitumor substance in only cancer cells under substantially anaerobic conditions.
Dependencies are inferred from the claim text.