Dna encoding ovine adenovirus (oav287) and its use as a viral vector
AssigneeCommonwealth Scientific and Industrial Research Organisation
InventorsGERALD WAYNE BOTH, DAVID BERNARD BOYLE, SUDHANSHU VRATI
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Abstract
A genome of an ovine adenovirus designated OAV287 is isolated from sheep and sequenced. Portions of the genome not essential for maintenance or viability of the virus can be deleted or altered. A nucleotide sequence encoding a non-adenoviral polypeptide can be incorporated into the genome. The a full-length clone of the genome can be provided as part of a plasmid or viral vector. Cells can be transformed with a vector of the invention such that they express an exogenous protein.
Claims23
An isolated DNA molecule comprising a nucleotide sequence encoding the genome of ovine adenovirus (OAV287) substantially as shown in FIG. 1 or a functionally equivalent nucleic acid sequence.
An isolated DNA molecule comprising a nucleic acid sequence encoding the genome of ovine adenovirus (OAV287) substantially as shown in FIG. 1 wherein a portion of the adenoviral genome not essential for the maintenance or viability of the native adenovirus is deleted or altered.
An isolated DNA molecule comprising at least a 15 nucleic acid base sequence being substantially unique to the ovine adenovirus (OAV287) nucleic acid sequence as shown in FIG. 1.
A viral vector comprising a DNA molecule including a nucleic acid sequence encoding the genome of ovine adenovirus (OAV287) substantially as shown in FIG. 1 or a functionally equivalent nucleic acid sequence or a portion thereof and at least one nucleic acid sequence encoding a non-adenoviral polypeptide or polypeptides.
A viral vector comprising a DNA molecule including a nucleic acid sequence encoding the genome of ovine adenovirus (OAV287) substantially as shown in FIG. 1 wherein a portion of the adenoviral genome not essential for the maintenance or viability of the native adenovirus is deleted or altered, and at least one nucleic acid sequence encoding a non-adenoviral polypeptide or polypeptides.
A viral vector comprising a DNA molecule including a nucleic acid sequence encoding the genome of ovine adenovirus (OAV287) substantially as shown in FIG. 1 or a functionally equivalent nucleic acid sequence or a portion thereof and at least one nucleic acid sequence encoding a functional RNA molecule.
Dependencies are inferred from the claim text.