Solution Phase Biopolymer Synthesis
AssigneeHubert Koster
InventorsHubert Koster, Ralph Worl
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Abstract
Multifunctional liquid phase carriers (LPCs) and methods of using LPCs for the preparation of biopolymers are provided. The LPCs are highly symmetrical compounds that possess more than two points of attachment for biopolymer synthesis. The LPCs have the formula Sp(X1)n, where Sp is a highly symmetrical moiety such that all X1 groups are equivalent. X1 is a functional group that is suitable for biopolymer synthesis, including OH, SH, NH2, COOH and the like. Biopolymers that may be produced using the methods provided include oligonucleotides, peptides, protein nucleic acids (PNAs) and oligosaccharides. Analogs of the biopolymers may also be prepared using the methods.
Claims49
A liquid phase carrier (LPC) of formula Sp(X1)n, wherein:
- Sp is a polyvalent group that has more than two points of attachment, n is the number of points of attachment in Sp and X1 is a reactive group for synthesis of biopolymers.
A method of solution phase biopolymer synthesis, comprising the steps of:
- (a) reacting an LPC of formula Sp(X1)n with a first monomer N1;
- (b) separating and purifying the product of step (a) to afford a compound of formula Sp(X1-N1)n;
- (c) reacting the product of step (b) with a second monomer N2, a dimer N2-N3 or a trimer N2-N3-N4;
- and
- (d) repeating steps (b) and (c) to produce an LPC-bound biopolymer of formula Sp(X1-N1-N2- . . . -Nm)n, where m is 3 to 100, wherein:
- Sp is a polyvalent group that has more than two points of attachment, n corresponds to the number of points of attachment in Sp and X1 is a reactive group for biopolymer synthesis;
- N1, N2, N3 . . . Nm are biopolymer monomers;
- and the dimers and trimers comprise the monomers.
A method of solution phase biopolymer synthesis, comprising the steps of:
- (a) reacting an LPC of formula Sp(X1)n with a first monomer N1;
- (b) separating and purifying the product of step (a) to afford a compound of formula Sp(X1-N1)n;
- (c) reacting the product of step (b) with a second monomer N2, a dimer N2-N3 or a trimer N2-N3-N4;
- and
- (d) repeating steps (b) and (c) to produce an LPC-bound biopolymer of formula Sp(X1-N1-N2- . . . -Nm)n, where m is 3 to 100, wherein:
- Sp is a polyvalent group that has two or more points of attachment, n corresponds to the number of points of attachment in Sp and X1 is a reactive group for biopolymer synthesis;
- N1, N2, N3 . . . Nm are biopolymer monomers;
- the dimers and trimers comprise the monomers;
- and
- the protocol used in steps (c) and (d) to synthesize the biopolymer, preferably the oligonucleotide, is the phosphoramidite protocol.
Dependencies are inferred from the claim text.