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US 2002/0002713 A1Published application
Starch branching enzyme IIb
OwnerStephen M. Allen, Diane M. Beckles +2inventors
InventorsStephen M. Allen, Diane M. Beckles, Karlene H. Butler, Richard W. Pearlstein
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Abstract
This invention relates to an isolated nucleic acid fragment encoding a starch branching enzyme. The invention also relates to the construction of a chimeric gene encoding all or a portion of the starch branching enzyme, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the starch branching enzyme in a transformed host cell.
Claims15
An isolated polynucleotide comprising:
- (a) a first nucleotide sequence encoding a first polypeptide having starch branching enzyme IIb activity, wherein the amino acid sequence of the first polypeptide and the amino acid sequence of SEQ ID NO:4 have at least 95% identity based on the Clustal alignment method,
- (b) a second nucleotide sequence encoding a second polypeptide having starch branching enzyme lib activity, wherein the amino acid sequence of the second polypeptide and the amino acid sequence of SEQ ID NO:2 have at least 97% identity based on the Clustal alignment method, or
- (c) the complement of the first or second nucleotide sequence.
An isolated polypeptide having starch branching enzyme IIb activity, wherein the polypeptide comprises:
- (a) a first amino acid sequence, wherein the first amino acid sequence and the amino acid sequence of SEQ ID NO:4 have at least 95% identity based on the Clustal alignment method, or
- (b) a second amino acid sequence, wherein the second amino acid sequence and the amino acid sequence of SEQ ID NO:2 have at least 97% identity based on the Clustal alignment method.
Dependencies are inferred from the claim text.