Novel nucleotide sequences encoding the gpm gene
AssigneeDegussa AG
InventorsBettina Mockel, Walter Pfefferle
Life of this patent
Estimating the term…
Abstract
The invention relates to an isolated polynucleotide which contains a polynucleotide sequence selected from the group comprising: a) a polynucleotide which is at least 70% identical to a polynucleotide coding for a polypeptide containing the amino acid sequence of SEQ ID NO:2, b) a polynucleotide coding for a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID NO:2, c) a polynucleotide which is complementary to the polynucleotides of a) or b), and d) a polynucleotide containing at least 15 consecutive bases of the polynucleotide sequence of a), b) or c), and to fermentation processes for the preparation of L-amino acids with amplification of the gpm gene.
Claims23
An isolated polynucleotide from corynebacteria which contains a polynucleotide sequence selected from the group consisting of:
- a) a polynucleotide which is at least 70% identical to a polynucleotide encoding a polypeptide containing the amino acid sequence of SEQ ID NO:
- 2,
- b) a polynucleotide encoding a polypeptide containing an amino acid sequence which is at least 70% identical to the amino acid sequence of SEQ ID NO:2,
- c) a polynucleotide which is complementary to the polynucleotides of a) or b), and
- d) a polynucleotide containing at least 15 consecutive bases of the polynucleotide sequence of a), b) or c).
A fermentation process for the preparation of an L-amino acid, comprising the following steps:
- a) fermentation of the L-lysine-producing corynebacteria in which at least the gpm gene or nucleotide sequences coding therefor are amplified and, in particular, overexpressed,
- b) enrichment of the L-amino acid in the medium or in the cells of the bacteria, and
- c) isolation of the L-amino acid.
Dependencies are inferred from the claim text.