Low cost manufacture of oligosaccharides
OwnerShawn Defrees, Karl Johnsoninventors
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Abstract
This invention provides recombinant cells, reaction mixtures, and methods that are useful for the enzymatic synthesis of product saccharides. The recombinant cells contain a heterologous gene that encodes a glycosyltransferase which catalyzes at least one step of the enzymatic synthesis, as well as a system for generating a nucleotide sugar that can serve as a substrate for the glycosyltransferase.
Claims71
A reaction mixture for producing a product saccharide, wherein the reaction mixture comprises an acceptor saccharide and a first type of plant or microorganism cell that produces:
- a) a nucleotide sugar, and b) a first recombinant glycosyltransferase that catalyzes the transfer of a sugar from the nucleotide sugar to the acceptor saccharide to form the product saccharide.
A cell that produces a product saccharide, wherein the cell comprises:
- a) a recombinant gene that encodes a glycosyltransferase;
- b) an enzymatic system for forming a nucleotide sugar that is a substrate for the glycosyltransferase;
- and
- c) an exogenous saccharide acceptor moiety;
- wherein the glycosyltransferase catalyzes the transfer of a sugar from the nucleotide sugar to the acceptor moiety to produce the product saccharide.
A cell that produces a sulfated polysaccharide, the cell comprising:
- a heterologous gene that encodes a sulfotransferase;
- and
- an enzymatic system that produces PAPS.
A method of producing a product saccharide, the method comprising contacting a microorganism or plant cell with an acceptor saccharide, wherein the cell comprises:
- a) an enzymatic system for forming a nucleotide sugar;
- and
- b) a recombinant glycosyltransferase which catalyzes the transfer of a sugar from the nucleotide sugar to the acceptor saccharide to produce the product saccharide.
Dependencies are inferred from the claim text.