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AssigneePrimaGen Holding B.V.
InventorsMarinus Petrus de Baar, Eveline Catherina A. C. Timmermans, Bob van Gemen
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The invention provides a method for reducing background in hybridization reactions of nucleic acids involving at least two homologous probes, wherein at least one of the probes is non-linear, or two homologous target sequences and a non-linear probe. Background is reduced by introducing an intended mismatch with a target sequence in at least one of the probes. The presence of the mismatch reduces the specificity of probes not entirely complementary to a target sequence to such an extent that the background signal is reduced. A set of mixed homologous probes, wherein at least one of the probes is non-linear, comprising such specific mismatch is also provided. The set can be used for the detection of variants of a family of nucleic acids, for instance a number of HIV variants. The invention also provides kits for carrying out the methods according to the invention.
A method for reducing background signals in a hybridization reaction of nucleic acids involving at least two homologous probes, wherein at least one of the two homologous probes is a non-linear probe, said method comprising:
A method for reducing background signals in a hybridization reaction of nucleic acids involving at least two homologous target sequences, said method comprising:
A set of mixed homologous probes for detecting at least one allelic variant of a nucleic acid, wherein at least one of said set of mixed homologous probes is non-linear, said set of mixed homologous probes comprise at least one sequence completely complementary to and specific for one of the allelic variants of said nucleic acid, except for a specific mismatch located upstream, downstream or both upstream and downstream from the site of variation.
A method of conducting a hybridization reaction comprising:
A kit for detecting at least one target sequence from a family of target sequences, said kit comprising at least one non-linear probe complementary to a target sequence specific for said family of target sequences and having a mismatch in a complementary sequence and further comprising suitable means for detection and/or amplification and/or isolation of nucleic acids.
Dependencies are inferred from the claim text.