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US 2002/0001801 A1Published application
Parallel genotyping of multiple patient samples
AssigneeIlluminia, Inc.
InventorsJian-Bing Fan, Mark S. Chee
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Abstract
The present invention relates to parallel genotyping (or other sample analysis) of multiple patients by direct sample immobilization onto microspheres of an array. The patient beads can then be used in a variety of target analyte analyses.
Claims26
An array composition comprising:
- a) a substrate with a surface comprising discrete sites;
- and
- b) a population of microspheres comprising at least a first and a second subpopulation,
- wherein the microspheres of each subpopulation each comprise a plurality of target analytes;
- wherein said microspheres are distributed on said surface.
2 more elements
The array composition according to claim 13, wherein said first and second target source are first and second patients, respectively.
A method comprising:
- a) providing an array composition comprising:
- i) a substrate with a surface comprising discrete sites;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation, wherein the microspheres of each subpopulation each comprise a plurality of target analytes;
- wherein said microspheres are distributed on said surface;
- b) contacting said array composition with a first set of readout probes;
- c) detecting the presence of a first target analyte.
4 more elements
A method of genotyping comprising:
- a) providing an array composition comprising:
- i) a substrate with a surface comprising discrete sites;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation, wherein the microspheres of each subpopulation each comprise at least first and second target analytes attached to said microspheres with first and second attachment moieties, respectively;
- wherein said microspheres are randomly distributed on said surface;
- b) contacting said array composition with a first set of extension probes that hybridize with at least said first target sequence adjacent to a first detection position to form an extension complex;
- c) contacting said extension complex with a composition comprising
- i) at least a first nucleotide;
- ii) polymerase;
- wherein said polymerase extends a first extension probe with said first nucleotide when said first nucleotide is complementary to said first detection position of said first target sequence;
- and
- c) detecting the presence of said first nucleotide.
9 more elements
A method of determining the identification of a nucleotide at a detection position in at least a first target sequence comprising:
- a) providing an array composition comprising:
- i) a substrate with a surface comprising discrete sites;
- and
- ii) a population of microspheres comprising at least a first and a second subpopulation, wherein the microspheres of each subpopulation each comprise a plurality of target sequences;
- b) forming a first hybridization complex between said first target sequence and at least a first readout probe;
- and
- c) determining the nucleotide at said detection position.
4 more elements
Dependencies are inferred from the claim text.