Diagnostic methods using serial testing of polymorphic loci
OwnerStanley N. Lapidusinventor
Life of this patent
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Abstract
Methods are provided for assaying the heterozygosity status of an individual member of a population. Methods of the invention are useful for detecting loss of heterozygosity in a nucleic acid sample. Methods of the invention are particularly useful for identifying individuals with mutations indicative of cancer.
Claims15
A method for detecting indicia of disease in a biological sample, the method comprising the steps of:
- (a) serially analyzing members of a plurality of polymorphic loci until a member of said plurality is determined to be a heterozygous locus;
- (b) determining a first number of a first allele of said heterozygous locus;
- (c) determining a second number of a second allele of said heterozygous locus;
- and
- (d) determining whether a statistically-significant difference exists between said first and second numbers, the presence of said statistically-significant difference being indicative of the presence of a disease.
A method for detecting a deletion in a biological sample, the method comprising the steps of:
- (a) serially analyzing members of a predetermined plurality of polymorphic loci until a member of said plurality is determined to be a heterozygous locus in said biological sample;
- (b) determining a first number of a first allele of said heterozygous locus;
- (c) determining a second number of a second allele of said heterozygous locus;
- and
- (d) determining whether a statistically-significant difference exists between said first and second numbers, the presence of said statistically-significant difference being indicative of the presence of a deletion.
A method for detecting an informative genetic locus in a biological sample, the method comprising serially analyzing individual members of a predetermined plurality of genetic loci until a member of said plurality that is heterozygous is identified.
Dependencies are inferred from the claim text.