Organism-compatible materials with combined extra-cellular matrices, extracellular-matrix preparations, and production methods
OwnerKikuji Yamashita, Hidemi Ukai +1inventors
InventorsKikuji Yamashita, Hidemi Ukai, Murakami Kouichi
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Abstract
This invention provides organism-compatible materials with combined extracellular matrices, which have high affinity with cells of organisms and are capable of hastening cell differentiation, and their production methods. An organism-compatible material with combined extracellular matrices is produced by letting cells of a region of an organism, to which the material is to be applied, form extracellular matrices on a calcification layer formed on a base. When it is applied to the region of the organism, it causes the migration, proliferation, and differentiation of necessary cells to reproduce a necessary tissue quickly.
Claims13
An organism-compatible material with combined extracellular matrices comprising a base made of a material for organisms, a calcification layer formed on the base, and extracellular matrices formed on the layer by cells of a region of an organism to which the organism-compatible material with combined extracellular matrices is to be applied
A production method of an organism-compatible material with combined extracellular matrices, wherein cells of a region of an organism, to which the material is to be applied, are cultured on a base made of titanium or a titanium alloy in a culture solution and, thereby, extracellular matrices are formed between a calcification layer formed on the base and the cells.
A production method of an organism-compatible material with combined extracellular matrices, comprising the steps of:
- culturing cells of a region of an organism, to which the material is to be applied, on a base made of titanium or a titanium alloy in a culture solution to form extracellular matrices between a calcification layer formed on the base and the cells;
- and
- removing the cells.
A production method of an organism-compatible material with combined extracellular matrices comprising the steps of:
- culturing cells of a region of an organism, to which the material is to be applied, on a base of titanium or a titanium alloy in a culture solution to form extracellular matrices between a calcification layer formed on the base and the cells;
- removing the cells;
- decalcifying the base with the calcification layer and the extracellular matrices to obtain suspension of the extracellular matrices;
- concentrating the suspension;
- and
- combining the extracellular matrices in the concentrated suspension with another base made of titanium or a titanium alloy.
An extracellular-matrix preparation for injection which is prepared from extracellular matrices formed by cells of a region of an organism, into which the preparation is to be injected, by concentrating and processing the extracellular matrices.
An extracellular-matrix ointment which is prepared from concentrated fluid of extracellular matrices formed by cells of a region of an organism, to which the ointment is to be applied, and an ointment base.
A production method of an extracellular-matrix preparation for injection comprising the steps of:
- culturing cells of a region of an organism, into which the preparation is to be injected, on a base of titanium or a titanium alloy in a culture solution to form extracellular matrices between a calcification layer formed on the base and the cells;
- removing the cells;
- decalcifying the base with the calcification layer and the extracellular matrices to obtain suspension of the extracellular matrices;
- concentrating the suspension by dialysis;
- sterilizing the concentrated suspension;
- and
- preparing the preparation for injection from the concentrated suspension.
A production method of an extracellular-matrix ointment comprising the steps of:
- culturing cells of a region of an organism, to which the ointment is to be applied, on a base of titanium or a titanium alloy in a culture solution to form extracellular matrices between a calcification layer formed on the base and the cells;
- removing the cells;
- decalcifying the base with the calcification layer and the extracellular matrices to obtain suspension of the extracellular matrices;
- concentrating the suspension;
- and
- adding an ointment base to the concentrated suspension to prepare the ointment from the concentrated suspension.
Dependencies are inferred from the claim text.