Zace1: a human metalloenzyme
OwnerPaul O. Sheppardinventor
Life of this patent
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Abstract
Angiotensin-converting enzyme is a zinc metallopeptidase that plays roles in blood pressure regulation and fertility. The catalytic activities of angiotensin converting enzymes include the production of the potent vasopressor angiotensin II from angiotensin I, and the inactivation of the vasodilatory peptide bradykinin. Zacel is a new form of human zinc metallopeptidase, which includes one zinc-dependent catalytic domain containing the motif “HEXXH” and one downstream “EX(I/V)X(D/S)” motif.
Claims22
An isolated polypeptide, comprising an amino acid sequence that is at least 70% identical to a reference amino acid sequence selected from the group consisting of:
- (a) amino acid residues 367 to 430 of SEQ ID NO:1,
- (b) amino acid residues 163 to 563 of SEQ ID NO:1,
- (c) amino acid residues 52 to 563 of SEQ ID NO:1,
- (d) amino acid residues 52 to 644 of SEQ ID NO:1,
- (e) amino acid residues 52 to 648 of SEQ ID NO:1,
- (f) amino acid residues 52 to 655 of SEQ ID NO:1,
- (g) amino acid residues 52 to 662 of SEQ ID NO:1,
- (h) amino acid residues 52 to 682 of SEQ ID NO:1,
- (i) amino acid residues 52 to 694 of SEQ ID NO:1, and
- (j) amino acid residues 1 to 694 of SEQ ID NO:1,
- wherein the isolated polypeptide either (a) specifically binds with an antibody that specifically binds with a polypeptide consisting of the amino acid sequence of SEQ ID NO:1, or (b) exhibits dipeptidyl carboxypeptidase activity.
A variant Zacel polypeptide, wherein the amino acid sequence of the variant polypeptide shares an identity with the amino acid sequence of SEQ ID NO:1 selected from the group consisting of at least 70% identity, at least 80% identity, at least 90% identity, at least 95% identity, or greater than 95% identity, and wherein any difference between the amino acid sequence of the variant polypeptide and the amino acid sequence of SEQ ID NO:1 is due to one or more conservative amino acid substitutions.
An isolated nucleic acid molecule that encodes polypeptide comprising an amino acid sequence selected from the group consisting of:
- (a) amino acid residues 367 to 430 of SEQ ID NO:1, (b) amino acid residues 163 to 563 of SEQ ID NO:1, (c) amino acid residues 52 to 563 of SEQ ID NO:1, (d) amino acid residues 52 to 644 of SEQ ID NO:1, (e) amino acid residues 52 to 648 of SEQ ID NO:1, (f) amino acid residues 52 to 655 of SEQ ID NO:1, (g) amino acid residues 52 to 662 of SEQ ID NO:1, (h) amino acid residues 52 to 682 of SEQ ID NO:1, (i) amino acid residues 52 to 694 of SEQ ID NO:1, and (j) amino acid residues 1 to 694 of SEQ ID NO:1.
An isolated polypeptide comprising at least 15 contiguous amino acid residues of the amino acid sequence of SEQ ID NO:1.
Dependencies are inferred from the claim text.