Homologous recombination-mediated transgene alterations in plants
AssigneeDekalb Genetics Corporation
InventorsDavid McElroy, David A. Walters, Larry A. Gilbertson
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Abstract
The invention provides a process to prepare a recombined transgenic Zea mays plant from a transgenic Zea mays plant, wherein the transgene in the recombinant plant has an altered genetic structure relative to the genetic structure of the transgene in the transgenic plant, due to homologous recombination-mediated transgene deletion, amplification or rearrangement.
Claims51
A method of preparing a transgenic cell having an altered transgene insertion comprising:
- a) obtaining a first transgenic cell, wherein the transgene insertion DNA sequence comprises a pre-selected DNA sequence flanked by directly repeated DNA sequences;
- b) obtaining a plurality of progeny cells of any generation of the first transgenic cell;
- c) selecting a progeny transgenic cell wherein at least a portion of the transgene insertion is altered as compared to the first transgenic cell.
A method of preparing a fertile transgenic plant having an altered transgene insertion comprising:
- a) obtaining a first transgenic plant, wherein the transgene insertion DNA sequence comprises a pre-selected DNA sequence flanked by directly repeated DNA sequences;
- b) obtaining a plurality of progeny of any generation of the first transgenic plant;
- c) selecting a progeny fertile transgenic plant wherein at least a portion of the transgene insertion is altered as compared to the first fertile transgenic plant.
A fertile transgenic plant wherein at least a portion of a transgene insertion is altered from a parent transgene insertion.
A progeny plant of any generation comprising an altered transgene insertion, wherein at least a portion of the transgene insertion is altered from the transgene insertion in a parental R0 plant.
An altered transgene insertion DNA sequence preparable by the method comprising:
- a) obtaining a first fertile transgenic plant comprising for a transgene insertion DNA sequence, wherein the transgene DNA sequence comprises a pre-selected DNA sequence flanked by directly repeated DNA sequences;
- b) obtaining a plurality of progeny of any generation of the first transgenic plant;
- and
- c) selecting a progeny fertile transgenic plant wherein at least a portion of the transgene insertion is altered as compared to the first fertile transgenic plant.
Dependencies are inferred from the claim text.