Urinary trypsin inhibitor assay containing a chelating agent
AssigneeBayer Aktiengesellschaft
InventorsGary E. Rehm, Michael J. Pugia, Paul F. Corey
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Abstract
Disclosed is an assay for determining the presence and concentration of trypsin inhibitor in urine samples. The assay reagents, which may be used either in the liquid or dry states, include trypsin, a trypsin substrate and a polycarboxylic chelating agent. The inclusion of the chelating agent in the assay has been found to reduce variation in the assay results.
Claims14
An assay for trypsin inhibitors in urine which comprises contacting a urine test sample with a buffered assay medium comprising trypsin, a substrate for trypsin which will produce a detectable response when cleaved by trypsin and a polycarboxylic chelating agent in sufficient quantity to inhibit interference with the assay from calcium present in the urine and correlating the concentration of trypsin inhibitor with the detectable response from the cleaving of the substrate.
A method for preparing a test device for the determination of trypsin inhibitor in urine which comprises contacting a pad of absorbant material with an aqueous solution of trypsin and a poly carboxylic chelating agent followed by drying the strip and contacting it with a solvent solution of a substrate for trypsin with subsequent drying.
Dependencies are inferred from the claim text.