Fermentation process for preparing erythritol by a high salt tolerant mutant of Candida sp.
AssigneeBolak Co., Ltd.
InventorsJin Ho Seo, Yeon Woo Ryu, Soo Ryun Jung, Sang Yong Kim
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Abstract
A fermentation process for preparing a high yield erythritol using a salt tolerant mutant of Candida sp. [Candida magnoliae SR101:KCCM-10160]. More specifically, the present invention relates to a process for preparing erythritol under optimal fermentation conditions for maximal erythritol production by optimizing the environmental conditions of culture, including medium component, pH, temperature, aeration rate and agitation speed.
Claims8
A fermentation process for production of erythritol using mutant cells of Candida magnoliae SR101 deposited to Korean Culture Center of Microorganism with accession number KCCM-10160 comprising the steps of:
- a) fermenting monosaccharide or disaccharide medium with cells by controlling following fermentation conditions:
- i) composition of medium for maximum production of erythritol consists of 10-50 (w/v) % of glucose, 0.2-2.0 (w/v) % of yeast extract, 0.1-10 (w/v) % of KH2P04, 0.1-5.0 (w/v) % of (N4)2S04 and 0.01-1.0 (w/v) % of MgS04.7H20,
- ii) pH of culture medium is 6-8,
- iii) temperature of cultivation is 26-30° C.,
- iv) aeration rate is 0.75-2.0 volume of air per volume of medium per minute, and
- v) agitation speed is 300-1200 rpm;
- b) feeding solution containing KCl continuously or intermittently fed into the culture broth during erythritol production phase to be 2-10% of its concentration;
- c) removing cells from the fermentation medium;
- and
- d) separating and recovering erythritol from the fermentation medium of step c).
The cells of Candida magnoliae SR101 [KCCM-10160].
A fermentation process for producing of erythritol using mutant cells of Candida magnoliae SR101 deposited to Korean Culture Center of Microorganism with accession number KCCM-10160 comprising:
- a) fermenting monosaccharide or disaccharide medium with cells of the Candida magnoliae SR101 deposited to Korean Culture Center of Microorganism;
- and
- b) feeding a solution containing KCl continuously or intermittently fed into the culture broth during erythritol production phase to be 2-10% of its concentration.
A fermentation process for producing of erythritol using mutant cells of Candida magnoliae SR101 deposited to Korean Culture Center of Microorganism with accession number KCCM-10160 comprising:
- a) fermenting monosaccharide or disaccharide medium with cells of the Candida magnoliae SR101 deposited to Korean Culture Center of Microorganism;
- and
- b) feeding a solution containing KCl continuously or intermittently fed into the culture broth.
The process of step 5 wherein the solution containing KCl is fed into the culture to be 2-10% of its concentration.
Dependencies are inferred from the claim text.