Polyhydroxyalkanoate synthase and gene encoding the same enzyme
OwnerTetsuya Yano, Takeshi Imamura +2inventors
InventorsTetsuya Yano, Takeshi Imamura, Sakae Suda, Tsutomu Honma
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Abstract
A novel polyhydroxyalkanoate (PHA) synthase derived from a microorganism capable of producing a PHA having a novel side-chain structure and a DNA encoding the amino acid sequence for the synthase are provided. Two PHA synthase proteins (SEQ ID NOs. 1 and 3) derived from Pseudomonas cichorii YN2 (FERM BP-7375) and PHA synthase genes encoding these PHA synthases are provided, respectively (SEQ ID NOs. 2 and 4). A recombinant microorganism is endowed with a PHA producing ability.
Claims12
A polyhydroxyalkanoate synthase having an amino acid sequence of SEQ ID NO. 1.
A polyhydroxyalkanoate synthase substantially retaining an amino acid sequence of SEQ ID NO. 1 and having a modified amino acid sequence in which amino acids are deleted, substituted or added as long as its polyhydroxyalkanoate synthase activity is not deteriorated.
The polyhydroxyalkanoate synthase gene comprising a DNA sequence of SEQ ID NO. 2 encoding the amino acid sequence of SEQ ID NO. 1.
A polyhydroxyalkanoate synthase having an amino acid sequence of SEQ ID NO. 3.
A polyhydroxyalkanoate synthase substantially retaining an amino acid sequence of SEQ ID NO. 3 and having a modified amino acid sequence in which amino acids are deleted, substituted or added as long as its polyhydroxyalkanoate synthase activity is not deteriorated.
The polyhydroxyalkanoate synthase gene comprising a DNA sequence of SEQ ID NO. 4 encoding the amino acid sequence of SEQ ID NO. 3.
Dependencies are inferred from the claim text.