Method of detecting a pathogen using a virus
AssigneeHer Majesty the Queen in right of Canada, as represented by the Minister of National Defence
InventorsJOHN W. CHERWONOGRODZKY, KAMIL LOTFALI
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Abstract
A bacteriophage linked to an enzyme can replace an antibody in a system for detecting the presence of a bacteria in a sample. Specifically Brucella abortus (a pathogen which causes brucellosis in cattle) can be detected using Brucella bacteriophage for the virus, urease for the enzyme linked to the bacteriophage, m-maleimidobenzoyl-N-hydrosysuccimide ester as a coupling reagent, sera from mice immunized with Brucella bacteriophage for a detector antibody, urease conjugated to anti-mouse sheep antibody for an indicator, and urea with bromcresol purple as the substrate. The materials can be used in indirect (sandwich) or direct enzyme-linked viral assays (ELVirA).
Claims7
A method of detecting the presence of a pathogenic bacteria in a liquid sample using a bacteriophage specific to the bacteria comprising the steps of producing a bacteriophage stock;
- conjugating the bacteriophage stock to an enzyme;
- mixing the conjugated bacteriophage with a sample suspected of containing the bacteria;
- and detecting any changes resulting from a reaction of the conjugated bacteriophage with the bacteria.
A method of detecting the presence of the bacteria Brucella abortus in a sample using virus anti-Brucella bacteriophage comprising the steps of producing a stock of Brucella bacteriophage, conjugating the Brucella bacteriophage to the enzyme urease;
- mixing the conjugated Brucella bacteriophage with a sample suspected of containing the bacteria Brucella abortus;
- and detecting any changes resulting from a reaction of the conjugated Brucella bacteriophage with the Brucella abortus.
Dependencies are inferred from the claim text.