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US 2001/0055779 A1Published application
Competition assay for detecting compound-protein interactions in situ
OwnerGlenna C. Burmerinventor
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Abstract
The described invention includes methods and assays to identify compounds that interact with a binding domain of a protein by a competition assay with a labeled ligand targeted to the peptides of the binding domain of that protein. These assays can be used for high throughput compound screening, or used to identify sites of compound interaction with proteins in tissue samples.
Claims18
A method for in situ detection of target-ligand binding in a biological sample, the method comprising the steps of:
- (a) incubating the in situ biological sample with a first potential ligand of interest;
- then
- (b) contacting the biological sample with a second ligand having a known binding specificity to a target protein present in the biological sample to create a second ligand-protein complex;
- (c) removing unbound second ligand from the second ligand-protein complex;
- and
- (d) detecting the level of binding of the second ligand to the target protein, wherein the level of binding of the second ligand to the target protein is proportional to the extent of competitive binding by the first ligand.
3 more elements
Dependencies are inferred from the claim text.