Patterned deposition of antibody binding protein for optical diffraction-based biosensors
AssigneeKimberly-Clark Worldwide, Inc.
InventorsKEVIN MCGRATH, ROSANN M. KAYLOR, DENNIS EVERHART
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Abstract
The present invention provides an inexpensive and sensitive device and method for detecting and quantifying analytes present in a medium. The device comprises a metalized film upon which is printed a specific, predetermined pattern of an antibody-binding proteins. Upon attachment of a target analyte to select areas of the plastic film upon which the protein is printed, diffraction of transmitted and/or reflected light occurs via the physical dimensions and defined, precise placement of the analyte. A diffraction image is produced which can be easily seen with the eye or, optionally, with a sensing device.
Claims56
A biosensor comprising:
- a polymer film;
- and
- an antibody-binding protein layer printed in a pattern onto the polymer film wherein the antibody-binding protein layer has an antibody thereon that is specific for an analyte.
A method of making a biosensor comprising printing a pattern of antibody-binding protein layer with a subsequent layer of antibody on the polymer film.
A method of detecting an analyte in a medium comprising;
- contacting the medium suspected of containing the analyte with a biosensing device, the biosensing device comprising:
- a polymer film;
- and an antibody-binding protein layer printed in a pattern onto the polymer film wherein the antibody-binding protein layer has a protein material thereon that is specific for the analyte;
- and
- transmitting a light through the polymer film;
- and
- detecting presence of the analyte bound to the protein material by detecting a pattern form by diffraction of the transmitted light.
A biosensor comprising:
- a polymer film;
- and
- an antibody-binding protein layer printed in a pattern onto the polymer film wherein the antibody-binding protein layer is capable of acting as a receptor for an analyte.
Dependencies are inferred from the claim text.