Post-labeling stabilization of radiolabeled proteins and peptides
AssigneeRhomed Incorporated
InventorsPaul O. Zamora, Michael J. Marek
Life of this patent
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Abstract
The subject invention relates to the use of ascorbic acid and derivatives thereof in stabilizing radiolabeled proteins and peptides against oxidation loss of radiolabel and autoradiolysis. Ascorbic acid is added after radiolabeling, including any required incubation period, but prior to patient administration.
Claims19
A method for stabilizing peptides radiolabeled with a radioisotope by addition of a stabilizer, selected from the group consisting of ascorbic acid and water soluble salts, esters and mixtures thereof to a composition containing a previously radiolabeled peptide, wherein said stabilizer is present in an amount sufficient to prevent oxidation loss and autoradiolysis of the radiolabeled peptide.
A method for stabilizing proteins radiolabeled with a radioisotope by addition of a stabilizer, selected from the group consisting of ascorbic acid and water soluble salts, esters and mixtures thereof to a composition containing a previously radiolabeled protein, wherein said stabilizer is present in an amount sufficient to prevent oxidation loss and autoradiolysis of the radiolabeled protein.
A composition for preparing a stable 99mTc-labeled anti-SSEA-1 IgM monoclonal antibody preparation, comprising:
- a preparation comprising a quantity of partially reduced anti-SSEA-1 IgM monoclonal antibody and stannous ion, wherein the anti-SSEA-1 IgM monoclonal antibody is labeled by the addition of 99mTc sodium pertechnetate and incubation;
- and a stabilizer selected from the group consisting of ascorbic acid and water soluble salts, esters and mixtures thereof wherein the stabilizer is added to the 99mTc-labeled anti-SSEA-1 IgM monoclonal antibody preparation subsequent to labeling the anti-SSEA-1 IgM monoclonal antibody by the addition of 99mTc sodium pertechnetate and incubation.
A composition stabilized against at least one of oxidation and autoradiolysis containing a radiolabeled peptide or a radiolabeled protein and a stabilizer selected from the group consisting of ascorbic acid and water-soluble salts and esters of ascorbic acid and mixtures of two or more of the foregoing said stabilizer having been added to said composition containing said peptide already radiolabeled.
A method for stabilizing a composition containing a radiolabeled peptide or a radiolabeled protein comprising the steps of:
- providing a composition containing said peptide or protein, said peptide or protein having already been radiolabeled, and adding to said composition a stabilizing agent selected from the group consisting of ascorbic acid and physiologically acceptable water-soluble salts and esters of ascorbic acid and mixtures of two or more of the foregoing said stabilizers in an amount effective to stabilize said radiolabeled peptide or protein against oxidation or autoradiolysis.
Dependencies are inferred from the claim text.