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US 2001/0044939 A1Published application
Small subunit of plant acetolactate synthase
OwnerLynn M. Abell, Saverio Carl Falco +1inventors
InventorsLynn M. Abell, Saverio Carl Falco, Omolayo O. Famodu
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Abstract
This invention relates to an isolated nucleic acid fragment encoding an acetolactate synthase small subunit. The invention also relates to the construction of a chimeric gene encoding all or a portion of the acetolactate synthase small subunit, in sense or antisense orientation, wherein expression of the chimeric gene results in production of altered levels of the acetolactate synthase small subunit in a transformed host cell.
Claims25
An isolated polynucleotide comprising:
- (a) a first nucleotide sequence encoding a first polypeptide comprising at least 35 amino acids, wherein the amino acid sequence of the first polypeptide and the amino acid sequence of SEQ ID NO:8 have at least 85% identity based on the Clustal alignment method,
- (b) a second nucleotide sequence encoding a second polypeptide comprising at least 100 amino acids, wherein the amino acid sequence of the second polypeptide and the amino acid sequence of SEQ ID NO:4, SEQ ID NO:20, or SEQ ID NO:22 have at least 80% identity based on the Clustal alignment method,
- (c) a third nucleotide sequence encoding a third polypeptide comprising at least 140 amino acids, wherein the amino acid sequence of the third polypeptide and the amino acid sequence of SEQ ID NO:14 have at least 80% identity based on the Clustal alignment method,
- (d) a fourth nucleotide sequence encoding a fourth polypeptide comprising at least 150 amino acids, wherein the amino acid sequence of the fourth polypeptide and the amino acid sequence of SEQ ID NO:10 or SEQ ID NO:12 have at least 80% identity based on the Clustal alignment method,
- (e) a fifth nucleotide sequence encoding a fifth polypeptide comprising at least 200 amino acids, wherein the amino acid sequence of the fifth polypeptide and the amino acid sequence of SEQ ID NO:6 have at least 80% identity based on the Clustal alignment method,
- (f) a sixth nucleotide sequence encoding a sixth polypeptide comprising at least 200 amino acids, wherein the amino acid sequence of the sixth polypeptide and the amino acid sequence of SEQ ID NO:18 have at least 85% identity based on the Clustal alignment method,
- (g) a seventh nucleotide sequence encoding a seventh polypeptide comprising at least 250 amino acids, wherein the amino acid sequence of the seventh polypeptide and the amino acid sequence of SEQ ID NO:16 have at least 80% identity based on the Clustal alignment method, or
- (h) the complement of the first, second, third, fourth, fifth, sixth, or seventh nucleotide sequence, wherein the complement and the first, second, third, fourth, fifth, sixth, or seventh nucleotide sequence contain the same number of nucleotides and are 100% complementary.
5 more elements
An isolated polypeptide comprising:
- (a) a first amino acid sequence comprising at least 35 amino acids, wherein the first amino acid sequence and the amino acid sequence of SEQ ID NO:8 have at least 85% identity based on the Clustal alignment method,
- (b) a second amino acid sequence comprising at least 100 amino acids, wherein the second amino acid sequence and the amino acid sequence of SEQ ID NO:4, SEQ ID NO:20 or SEQ ID NO:22 have at least 80% identity based on the Clustal alignment method,
- (c) a third amino acid sequence comprising at least 140 amino acids, wherein the third amino acid sequence and the amino acid sequence of SEQ ID NO:14 have at least 80% identity based on the Clustal alignment method,
- (d) a fourth amino acid sequence comprising at least 150 amino acids, wherein the fourth amino acid sequence and the amino acid sequence of SEQ ID NO:10 or SEQ ID NO:12 have at least 80% identity based on the Clustal alignment method,
- (e) a fifth amino acid sequence comprising at least 200 amino acids, wherein the fifth amino acid sequence and the amino acid sequence of SEQ ID NO:6 have at least 80% identity based on the Clustal alignment method,
- (f) a sixth amino acid sequence comprising at least 200 amino acids, wherein the sixth amino acid sequence and the amino acid sequence of SEQ ID NO:18 have at least 85% identity based on the Clustal alignment method, or
- (g) a seventh amino acid sequence comprising at least 250 amino acids, wherein the seventh amino acid sequence and the amino acid sequence of SEQ ID NO:16 have at least 80% identity based on the Clustal alignment method.
4 more elements
Dependencies are inferred from the claim text.