Methods for producing transgenic animals

A method for producing a transgenic animal comprising the steps of:

• transferring transgenic embryos, which were produced by transferring exogenous DNA to oocytes by injection of a retroviral vector, fertilizing the oocytes by intracytoplasmic sperm injection, and culturing said oocytes to the embryonic stage, to the oviducts of surrogate females;
• and
• producing a transgenic animal by parturition.

A method for producing a transgenic primate comprising the steps of:

• transferring said transgenic embryos which were produced by dissociating blastomeres from an embryo, removing the nuclei from the blastomeres, injecting the blastomere nuclei into enucleated oocytes by intracytoplasmic nuclear injection, and activating and culturing oocytes to an embryonic stage, to the oviducts of surrogate females;
• and
• producing a transgenic primate by parturition.

A method for producing a transgenic primate comprising the steps of:

• transferring transgenic embryos, which were produced by isolating nuclei from somatic cells, injecting the nuclei into enucleated oocytes by intracytoplasmic nuclear injection, then activating and culturing the oocytes to an embryo stage, to the oviduct of surrogate females;
• and
• producing a transgenic primate by parturition.

The method of claim 74, wherein said oocyte activation is selected from the group comprising chemical activation, sperm cytosolic (oscillin) activation, and electrical activation.

A method for producing a transgenic primate comprising the steps of:

• transferring transgenic embryos, which were produced by fertilizing an oocyte by intracytoplasmic sperm injection, then transferring exogenous DNA to the pronucleus of fertilized oocyte by pronuclear injection, then culturing the fertilized oocyte to an embryonic stage, to oviduct of surrogate females;
• and
• producing a transgenic primate by parturition.

A method of producing transgenic primate cells wherein said transgenic cells are used to treat human diseases.