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OwnerHirobumi Akoi, Harumi Kamachiinventors
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The present invention relates to a novel Rhodococcus bacterium and to a process of hydrolyzing a cyano group of a nitrile compound using a novel Rhodococcus bacterium to produce the corresponding carboxylic acid. The present invention also relates to a process of producing carboxylic acids, in particular cyano carboxylic acids using a transformant transformed with a plasmid containing a nitrilase gene, a nitrile hydratase gene and an amidase gene derived from Rhodococcus bacteria capable of exhibiting particularly excellent position selectivity for the cyano group of aromatic polynitrile compounds, to such a transformant, such a plasmid, to such genes, to a process of producing an enzyme using the transformant, and to enzymes obtained by the process. The carboxylic acids, in particular cyano carboxylic acids obtained by the present invention are useful as starting materials for the synthesis of drugs, agrochemicals, dyestuff and other chemicals.
A process for producing carboxylic acid, comprising converting at least one cyano group of a nitrile compound into a carboxyl group using a microorganism, wherein a variant microorganism defective or reduced in the activity of converting a cyano group into an amide group is used:
A variant microorganism having the activity of converting a cyano group into a carboxyl group and being defective or reduced in the activity of converting a cyano group into an amide group.
A Rhodococcus sp. SD826 (FERM BP-7305) strain.
A process for producing carboxylic acid, comprising converting a cyano group of a nitrile compound into a carboxyl group using a transformant transformed with a plasmid containing a nitrilase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequence shown by SEQ ID NO 2 of the sequence list.
A process for producing carboxylic acid, comprising converting a cyano group of a nitrile compound into a carboxyl group using a transformant transformed with a plasmid containing a nitrilase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 1 in the sequence list.
A nitrilase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequence shown by SEQ ID NO 2 of the sequence list.
A nitrilase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 1 of the sequence list.
A process for producing amide compound, comprising converting a cyano group of a nitrile compound into an amide group using a transformant transformed with a plasmid containing a nitrile hydratase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequences shown by SEQ ID NOs 4 and 5 of the sequence list.
A process for producing amide compound, comprising converting a cyano group of a nitrile compound into an amide group using a transformant transformed with a plasmid containing a nitrile hydratase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 3 in the sequence list.
A process for producing carboxylic acid, comprising converting an amide group of an amide compound into a carboxyl group using a transformant transformed with a plasmid containing an amidase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequence shown by SEQ ID NO 7 of the sequence list.
A process for producing carboxylic acid, comprising converting an amide group of an amide compound into a carboxyl group using a transformant transformed with a plasmid containing an amidase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 6 of the sequence list.
A process for producing carboxylic acid, comprising converting a cyano group of a nitrile compound into a carboxyl group using a transformant transformed with a plasmid containing both a nitrile hydratase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequences shown by SEQ ID NOs 4 and/or 5 of the sequence list and an amidase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequence shown by SEQ ID NO 7 of the sequence list.
A process for producing carboxylic acid, comprising converting a cyano group of a nitrile compound into a carboxyl group using a transformant transformed with a plasmid containing both a nitrile hydratase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 3 of the sequence list and an amidase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 6 of the sequence list.
A nitrile hydratase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequence shown by SEQ ID NOs 4 and/or 5 of the sequence list.
A nitrile hydratase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 3 of the sequence list.
An amidase gene derived from Rhodococcus bacterium consisting of a DNA sequence encoding the amino acid sequence shown by SEQ ID NO 7 of the sequence list.
An amidase gene derived from Rhodococcus bacterium consisting of a DNA sequence shown by SEQ ID NO 6 of the sequence list.
Dependencies are inferred from the claim text.