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AssigneePhage Biotechnology Corporation
InventorsVitaliy A. Kordyum, Svitlana I. Chernykh, Iryna Yu Slavchenko, Oleksandr F. Vozlanov
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This invention relates to a method for enhancing the production of biologically active proteins and peptides in bacterial cells by infecting bacterial cells of the producer strain, which contain a plasmid with one or more targeted genes, with bacteriophage λ with or without the targeted gene(s). The phage increases synthesis of the targeted protein and induces lysis of the producer strain cells. Super-production is achieved by cultivating the producer strain cells under culture conditions that delay lytic development of the phage. The biologically active proteins and peptides subsequently accumulate in a soluble form in the culture medium as the cells of the producer strain are lysed by the phage.
A method for producing a biologically active protein, comprising:
A method for producing a biologically active protein, comprising:
A bacterial host cell with a plasmid having at least one copy of an expressible heterologous gene encoding a protein, wherein said host cell is infected with a bacteriophage capable of mediating lysis and also capable of lytic growth without lysis.
A strain of E. coli with a plasmid having at least one copy of an expressible heterologous gene encoding a protein, wherein said strain of E. coli is infected with bacteriophage λ having cI857, Qam 117 and Ram 54 mutations.
A strain of E. coli with a plasmid having at least one copy of an expressible heterologous gene encoding a protein, wherein said strain of E. coli is infected with bacteriophage λ having cI857, Qam 117 and Ram 54 mutations and at least one copy of an expressible heterologous gene encoding said protein.
Dependencies are inferred from the claim text.