Methodology of using raman imaging microscopy for evaluating drug action within living cells
OwnerJian Ling, Steven D. Weitman +1inventors
InventorsJian Ling, Steven D. Weitman, Michael A. Miller
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Abstract
A method of using Raman imaging microscopy to evaluate drug actions in living cells is disclosed. Specifically the invention describes the methods of using Raman imaging microscopy to detect drug uptake, distribution, binding, and metabolism in a single cell, and to study drug pharmacokinetics at the cellular level. The method involves measuring the Raman image of both the drug and the cell. Control images and post-treatment images of the cell were studied. Ratio images were calculated and the requisite information was obtained from a study of the intensity of the bright areas in the ratio images.
Claims14
A method of using Raman imaging microscopy for evaluating drug action within living cells comprising the steps of:
- first measuring a Raman spectrum of said drug to determine a pretreatment fingerprint of said drug;
- second measuring a Raman spectrum of said living cells in order to determine a pretreatment fingerprint of said living cells;
- treating a culture of said living cells with said drug to obtain treated living cells;
- next measuring a Raman spectrum of said treated living cells to obtain post-treatment images of said living cells;
- processing said post-treatment images and pretreatment fingerprints to obtain processed post-treatment images and processed pretreatment fingerprints;
- and
- dividing said post-treatment images by said processed pretreatment fingerprints to obtain a ratio of images which indicate the changes of said living cells after said treating whereby said changes are used to determine said drug action.
Dependencies are inferred from the claim text.