Stabilized coenzyme solutions and their use for determining dehydrogenases or substrates thereof
OwnerRalf Zielenskiinventor
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Abstract
Stabilized aqueous solution of a coenzyme for hydrogen-transferring enzymes characterized in that the solution contains NAD, NADP or a derivative thereof in an oxidized or reduced form and one or several organic compounds or salts thereof having a pKa value between 1.5 and 6.0 and/or a nitrogen compound of the general formula (I) 1 in which the residues R1, R2 and R3 are the same or different and denote hydrogen, or a saturated or unsaturated alkyl or aryl group as well as the use of the solution to determine dehydrogenases in particular lactate dehydrogenase or substrates thereof.
Claims17
Stabilized aqueous solution of a coenzyme for hydrogen-transferring enzymes characterized in that the solution contains NAD, NADP or a derivative thereof in an oxidized or reduced form and one or several organic compounds or salts thereof having a pKa value between 1.5 and 6.0 and/or a nitrogen compound of the general formula (I)
- 3
- in which the residues R1, R2 and R3 are the same or different and denote hydrogen, or a saturated or unsaturated alkyl or aryl group.
Kit for determining a hydrogen-transferring analyte in a sample comprising the following components:
- a first reagent which contains a dehydrogenase in a suitable system buffering between pH 8.5 and 10.0
- and
- a second reagent which contains a coenzyme for hydrogen-transferring enzymes and an organic compound having a pKa value between 1.5 and 6.0 and/or a nitrogen compound of the general formula (I)
- 4
- in which the residues R1, R2 and R3 are the same or different and denote hydrogen, or a saturated or unsaturated alkyl or aryl group.
Dependencies are inferred from the claim text.