Biopolymer arrays and their fabrication
AssigneeAgilent Technologies, Inc.
InventorMichel G. M. Perbost
Life of this patent
Estimating the term…
Abstract
A method of fabricating an addressable array of biopolymers on a substrate using a biomonomer with a first linking group which must be activated for linking to a substrate bound moiety, and apparatus and computer program products for executing the method. The method includes forming on a region of the substrate carrying the substrate bound moiety, a solid activator composition. A biomonomer containing fluid composition is deposited on the region so that the solid activator activates the first linking group and the biomonomer links to the substrate bound moiety. The foregoing steps may be repeated, wherein a biomonomer deposited and linked to the substrate bound moiety in one cycle is the substrate bound moiety for the next cycle, so as to form the biopolymer.
Claims33
A method of fabricating an addressable array of biopolymers on a substrate using a biomonomer with a first linking group which must be activated for linking to a substrate bound moiety, comprising:
- (a) forming on a region of the substrate carrying the substrate bound moiety, a solid activator composition;
- (b) depositing a biomonomer containing fluid composition on the region so that the solid activator activates the first linking group and the biomonomer links to the substrate bound moiety;
- and
- (c) repeating steps (a) and (b), wherein a biomonomer deposited and linked to the substrate bound moiety in one cycle is the substrate bound moiety for the next cycle, so as to form the biopolymer.
A method of fabricating an addressable array of biopolymers on a substrate using biomonomers each with a first linking group which must be activated for linking to a substrate bound moiety, and a deposition system with a head having multiple pulse jets each of which can dispense droplets of a fluid onto a substrate, each jet including a chamber with an orifice, and including an ejector which, when activated, causes a droplet to be ejected from the orifice, the method comprising:
- (a) depositing onto a region of the substrate carrying the substrate bound moiety, a fluid composition of a solid activator;
- (b) allowing fluid of the composition to evaporate to form the solid activator on the region;
- (c) then depositing from a pulse jet onto the region, a droplet of a biomonomer containing fluid composition so that the solid activator activates the first linking group and the biomonomer links to the substrate bound moiety;
- (d) repeating steps (a) through (c) at each of multiple regions of the substrate, wherein at each region a biomonomer deposited and linked to the substrate bound moiety in one cycle is the substrate bound moiety for the next cycle, so as to form a biopolymer at each of the multiple regions.
An apparatus for fabricating an addressable array of biopolymers on a substrate according to a target pattern, comprising:
- (a) a deposition system which can separately dispense onto a substrate, fluid compositions of different biomonomers each with a first linking group which must be activated for linking to a substrate bound moiety, and a fluid composition of a solid activator;
- (b) a processor to operate the deposition system, which processor derives from the target array pattern a target drive pattern for operating the deposition system to form the array, the target drive pattern including instructions to the deposition system to deposit the fluid composition of solid activator at each region at which a biomonomer monomer is to be deposited, separate from and preceding deposition of the biomonomer.
A computer program product, for use on an apparatus for fabricating an addressable array of biopolymer probes on a substrate according to a target array pattern, the program product comprising:
- a computer readable storage medium having a computer program stored thereon which, when loaded into a computer of the apparatus performs the steps of:
- deriving from the target array pattern a target drive pattern for operating a deposition system of the apparatus to form the array, the target drive pattern including instructions to the deposition system to deposit the fluid composition of solid activator at each region at which a biomonomer monomer is to be deposited, separate from and preceding deposition of the biomonomer.
Dependencies are inferred from the claim text.