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US 2001/0043916 A1Published application
Method using filtration aids for the separation of virus vectors from nucleic acids and other cellular contaminants
OwnerDavid S. McNeilly, William O. Osburninventors
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Abstract
Methods are disclosed for the purification of encapsulated viruses. The methods are advantageous in that they employ filtration aids, together with low concentrations of metal ions, in place of nucleases for purification. This provides important advantages for commercial scale purification of viruses.
Claims30
A method for purification of encapsulated viruses from cell culture, said method comprising:
- (a) lysing a cell culture containing encapsulated virus;
- (b) subjecting the composition resulting from step (a) to filtration with a substance selected from the group consisting of Diatomaceous Earth (DE) and poly-anionic cellulose filter aids, to generate a filtrate;
- (c) subjecting the filtrate of step (b) to one or more suitable concentration and diafiltration steps to generate concentrated and diafiltered retentate;
- and
- (d) subjecting the concentrated or diafiltered retentate of step (c) to one or more suitable purification steps and collecting a purified composition containing encapsulated viruses, wherein steps (c) and steps (d) may take place iteratively.
2 more elements
Dependencies are inferred from the claim text.