gamma-IFN liquid-droplet aerosol and method

A liquid-droplet aerosol composition for delivery to a patient's respiratory tract

• (a) formed by placing an aqueous γ-IFN solution having a known, selected γ-IFN biological activity, and containing a stabilizing agent and a dispersing agent, against a plate having defined-size openings, and forcing the solution through said openings under conditions effective to form aqueous droplets having (a) a volume mean diameter in a selected size range selected from the group consisting of (i) less than 1 microns, (ii) 1-3 microns, (iii) 3-5 microns, (iv) 5-10 microns, (v) greater than 10 microns, and (vi) two or more of the size ranges, (b) a γ-IFN biological activity substantially the same as that of the solution, and (c) a γ-IFN molecular size distribution substantially the same as that of the solution;
• and
• (b) characterized by an aerosol of aqueous droplets having (a) a volume mean diameter in a selected size range selected from the group consisting of (i) less than 1 microns, (ii) 1-3 microns, (iii) 3-5 microns, (iv) 5-10 microns, (v) greater than 10 microns, and (vi) two or more of the size ranges, (b) a g-IFN biological activity substantially the same as that of the solution, and (c) a g-IFN molecular size distribution substantially the same as that of the solution.