US 2001/0042718 A1 · STATIC SEPARATION METHOD USING NON-POROUS CELLULOSE BEADS

Abstract

An affinity separation method and system comprising an affinity separation media with low porosity and low non-specific binding, and a fluid containing a target compound to be isolated which is capable of binding onto the affinity separation media in a fluid mixing loop in a static filtration apparatus. The static filtration apparatus comprises an intermixing-chamber containing a filtration medium wherein a tangential flow is created for intermixing the affinity separation media and target compound in the fluid. The fluid is capable of passing through the filtration medium while the affinity separation media are substantially incapable of passing through the filtration medium. The affinity separation media are separated from the fluid by opening the filtrate outlet so as to allow the fluid to pass through the filtration medium of the static filtration apparatus. The filtrate can be thereby rendering substantially free of the target compound.

Claims38

Dependent claims

A method of separation for isolating a target compound from a fluid comprising:

(a) forming a suspension comprising
i) a fluid containing a target compound to be isolated, and
ii) a plurality of low porosity affinity particles capable of binding the target compound,
(b) maintaining said low porosity affinity particles and said fluid in contact for a sufficient period of time for binding of said target compound onto said low porosity affinity particles to be effected,
(c) introducing said suspension into a static filtration apparatus having a filtration medium,
(d) creating a tangential flow in said static apparatus thereby aiding in separation between said fluid and said low porosity affinity particles with the target compound attached to the surface thereof,
(e) passing said fluid devoid of the target compound through said filtration medium of said static filtration apparatus,
(f) washing said low porosity affinity particles, and
(g) eluting said target compound from said low porosity affinity particles.

6 more elements

Dependencies are inferred from the claim text.

Static separation method using non-porous cellulose beads

Life of this patent

Static separation method using non-porous cellulose beads

Life of this patent

Abstract

Claims38