Motilin homologs
AssigneeZymoGenetics, Inc.
InventorsPaul O. Sheppard, Theresa A. Deisher, Paul D. Bishop, Stephen R. Jaspers, Virender M. Labroo
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Abstract
The present invention is directed to polynucleotides, polypeptides and peptide fragments thereof, and uses thereof for a novel cDNA sequence which has homology to motilin. Tissue distribution of the mRNA for the novel polypeptide is specific to the stomach, small intestine and pancreas. The present invention further includes agonists, antagonists, antibodies, host cells expressing the cDNA encoding the novel motilin homologs and methods for increasing gastric motility using the novel molecules.
Claims18
An isolated polynucleotide molecule encoding a polypeptide selected from the group consisting of:
- (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 70 to nucleotide 111;
- (b) allelic variants of (a);
- (c) orthologs of (a) and (b);
- and
- (d) degenerate nucleotide sequences of (a), (b) or (c).
An isolated polynucleotide molecule encoding a polypeptide selected from the group consisting of:
- (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 70 to nucleotide 120;
- (b) allelic variants of (a);
- (c) orthologs of (a) or (b);
- and
- (d) degenerate nucleotide sequences of (a), (b) or (c).
An isolated polynucleotide molecule encoding a polypeptide selected from the group consisting of:
- (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 70 to nucleotide 351;
- (b) allelic variants of (a);
- (c) orthologs of (a) or (b);
- and
- (d) degenerate nucleotide sequences of (a), (b) or (c).
An isolated polynucleotide molecule encoding a polypeptide selected from the group consisting of:
- (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 1 to nucleotide 111;
- (b) allelic variants of (a);
- (c) orthologs of (a) or (b);
- and
- (d) degenerate nucleotide sequences of (a), (b) or (c).
An expression vector comprising the following operably linked elements:
- a transcription promoter;
- a DNA segment selected from the group consisting of:
- (a) polynucleotide molecules comprising a nucleotide sequence as shown in SEQ ID NO:
- 1 from nucleotide 70 to nucleotide 111;
- (b) allelic variants of (a);
- (c) orthologs of (a) or (b);
- and
- (d) degenerate nucleotide sequences of (a), (b) or (c);
- a transcription terminator.
An isolated polypeptide selected from the group consisting of:
- (a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 24 to residue 37;
- (b) allelic variants of (a);
- and
- (c) orthologs of (a) or (b).
An isolated polypeptide selected from the group consisting of:
- (a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 24 to 41;
- (b) allelic variants of (a);
- and
- (c) orthologs of (a) or (b).
An isolated polypeptide selected from the group consisting of:
- (a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 24 to residue 117;
- (b) allelic variants of (a);
- and
- (c) orthologs of (a) or (b).
An isolated polypeptide selected from the group consisting of:
- (a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 1 to residue 37;
- (b) allelic variants of (a);
- and
- (c) orthologs of (a) or (b).
An antibody that binds to an epitope of a polypeptide selected from the group consisting of:
- (a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 24 to residue 117;
- (b) allelic variants of (a);
- and
- (c) orthologs of (a) or (b).
A method of stimulating gastric motility comprising administering to a mammal in need thereof, an amount of a pharmaceutical composition comprising an isolated polypeptide selected from the group consisting of:
- (a) polypeptide molecules comprising an amino acid sequence as shown in SEQ ID NO:
- 2 from residue 24 to residue 37;
- (b) allelic variants of (a);
- and
- (c) orthologs of (a) or (b);
- sufficient to increase transit time or gastric emptying of an ingested substance.
Dependencies are inferred from the claim text.