Cocoa sphingolipids, cocoa extracts containing sphingolipids and methods of making and using same
OwnerHarold H. Schmitz, Sheryl Lazarus +1inventors
InventorsHarold H. Schmitz, Sheryl Lazarus, John F. Hammerstone
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Abstract
The invention relates to cocoa sphingolipids, cocoa extracts containing cocoa sphingolipids, and compositions containing cocoa sphingolipids and/or metabolic derivatives thereof. The invention also relates to methods of isolating and purifying sphingolipids and methods of using the cocoa sphingolipids and/or metabolic derivatives thereof.
Claims28
A sphingolipid-containing cocoa extract which is substantially free of cocoa polyphenols.
The sphingolipid-containing cocoa extract obtained by extracting polar lipids from a cocoa material.
A sphingolipid-containing cocoa extract which is prepared by a process comprising the steps of:
- (i) extracting polar lipids from a cocoa material to obtain a crude sphingolipid-containing extract;
- (ii) hydrolyzing said crude sphingolipid-containing extract with a base;
- and
- (iii) removing the products of said base hydrolysis.
A sphingolipid-containing cocoa extract prepared by a process comprising the steps of:
- (i) extracting a cocoa material with butanol to obtain a crude extract containing sphingolipids;
- (ii) extracting the crude extract with hexane to obtain a hexane-purified cocoa extract;
- (iii) hydrolyzing the hexane-purified cocoa extract with a base to obtain a base-hydrolyzed cocoa extract;
- and
- (iv) extracting the base-hydrolyzed cocoa extract with chloroform to remove the products of base hydrolysis.
A sphingolipid selected from the group consisting of d81:2Δ4,Δ8/c16:0;
- d18:2Δ4,Δ8/c18:0;
- d18:2Δ4,Δ8/c20:0;
- d18:2Δ4,Δ8/h16:0;
- d18:2Δ4,Δ8/h18:0;
- d18:2Δ4,Δ8/h20:0;
- d18:2Δ4,Δ8/h22:0;
- d18:2Δ4,Δ8/24:0;
- t18:1Δ8/h20:0;
- t18:1Δ8/h22:0;
- t18:1Δ8/h24:0;
- t18:1Δ8/h26:0;
- and t18:1Δ8/h28:0.
A method for extracting and purifying sphigolipids from a sphingolipid-containing material comprising the steps of:
- (i) extracting polar lipids from a sphingolipid-containing material to obtain a crude extract;
- and
- (ii) extracting the crude extract with hexane to obtain a hexane-purified extract.
A method of altering cell proliferation by contacting the cell with the sphingolipid-containing cocoa extract, a cocoa sphingolipid or a metabolic derivative thereof.
A method of treating cancer by administering to a mammal a composition comprising a sphingolipid-containing cocoa extract, a cocoa sphingolipid or a metabolic derivative thereof.
Dependencies are inferred from the claim text.