Haloperoxidases with altered pH profiles
AssigneeNovozymes A/S
InventorsAllan Svendsen, Louise Jorgensen
Life of this patent
Estimating the term…
Abstract
The present invention relates to variants of a parent vanadium-containing haloperoxidase, which variant has haloperoxidase activity and an altered pH optimum and comprises a mutation in a position corresponding to at least one of the following positions: R490A,L,I,Q,M,E,D; A399G; F397N,Y,E,Q; P395A,S; R360A,L,I,Q,M,E,D; K353Q,M; S402A,T,V,S; D292L; A501S; W350F,Y; V495A,T,V,S; K394 A,L,I,Q,M,E,D; wherein the parent haloperoxidase has the amino acid sequence given in SEQ ID No. 1, or the parent haloperoxidase has an amino acid sequence which is at least 80% homologous to SEQ ID No. 1.
Claims19
A DNA sequence encoding a variant having haloperoxidase activity, an altered pH optimum compared with a haloperoxidase, and an amino acid sequence that differs from the amino acid sequence of the haloperoxidase, wherein the amino acid sequence of the haloperoxidase is at least 80% homologous with the haloperoxidase of SEQ ID NO:1 and the difference between the amino acid sequence of the variant and the amino acid sequence of the haloperoxidase comprises:
- (a) a different amino acid at position 292 wherein the different amino acid is L or E;
- (b) a different amino acid at position 350 wherein the different amino acid is F or Y;
- (c) a different amino acid at position 353 wherein the different amino acid is Q or M;
- (d) a different amino acid at position 360 selected from the group consisting of A, L, I, Q, M, E and D;
- (e) a different amino acid at position 394 selected from the group consisting of A, L, I, Q, M, E and D;
- (f) a different amino acid at position 395 wherein the different amino acid is A or S;
- (g) a different amino acid at position 397 selected from the group consisting of N, Y, E, and Q;
- (h) a different amino acid at position 399 wherein the different amino acid is G;
- (i) a different amino acid at position 402 selected from the group consisting of A, T, and V;
- (j) a different amino acid at position 490 selected from the group consisting of A, L, I, Q, M, E, and D;
- (k) a different amino acid at position 495 selected from the group consisting of A, T, and S;
- and/or
- (l) a different amino acid at position 501 wherein the different amino acid is S,
- wherein each position corresponds to the position of the amino acid sequence of the haloperoxidase of SEQ ID NO:1.
Dependencies are inferred from the claim text.