Gene coded for interleukin-2 polypeptide, recombinant DNA carrying the said gene, a living cell line possessing the recombinant DNA, and method for producing interleukin-2 using the said cell
AssigneeAjinomoto Co., Inc.
InventorsTadatsugu Taniguchi, Masami Muramatsu, Haruo Sugano, Hiroshi Matsui, Nobukazu Kashima, Junji Hamuro
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Abstract
A gene coded for a polypeptide which possesses interleukin-2 is isolated, and connected with a vector DNA which is capable of replicating in a procaryotic or eucaryotic cell at a position downstream of a promoter gene in the vector obtaining a recombant DNA, with which the cell is transformed to produce interleukin-2.
Claims127
A cloned gene coded for a polypeptide possessing the activity of interleukin-2.
A DNA, prepared recombinantly which comprises a gene coded for a polypeptide which possesses the activity, of inter-leukin-2, and a vector DNA capable of propagating in a procaryotic or eucaryotic the coding sequence of said gene being located at a position downstream of a promoter sequence.
The recombinant DNA of clam 36, wherein the base sequence of said gene ends at poly (A) in FIG. 2 (a).
A cell of eucarlote or procaryote transformed with a recombinant DNA comprising a gene coding for a polypeptide possessing the activity of interleukin-2 and a vector DNA capable of propagating in said cell, and the coding sequence of said gene being located at a position downstream of a promoter sequence.
A method for producing interleukin-2 which comprises culturing aleucaryotic or procaryotic cell culture medium, transformed with a recombinant DNA to produce interleukin-2 and recovering the produced interleukin-2;
- said recombinant DNA comprising a gene coded for a polypeptide which posesses the activity of interleukin-2 and a vector DNA which is capable of replicating in said cell, and the coding sequence of said gene being located at a position downstream of a promoter sequence.
Dependencies are inferred from the claim text.