Method for enhanced protein stabilization and for production of cell lines useful for production of such stabilized proteins

A method for production of cell lines exhibiting permanent genetic modification, said method comprising the steps of:

• selecting and propagating permissive eucaryotic cells;
• introducing a transfection reagent to said permissive eucaryotic cells to produce transfected eucaryotic cells;
• incubating said transfected eucaryotic cells;
• and
• selecting from said transfected eucaryotic cells at least one first cell line exhibiting high yield of a protein expressed as a result of transfection by said transfection reagent.

A viral agent produced by the steps of:

• selecting and propagating permissive eucaryotic cells;
• transiently stressing said cells for a period of time to produce stressed eucaryotic cells exhibiting increased production of at least one stress protein;
• introducing a virus stock to infect said stressed eucaryotic cells;
• incubating said infected stressed eucaryotic cells;
• and
• harvesting the resultant viral agent produced by said infected stressed eucaryotic cells.

A cell line exhibiting permanent genetic modification produced by the steps of:

• selecting and propagating permissive eucaryotic cells;
• introducing a transfection reagent to said permissive eucaryotic cells to produce transfected eucaryotic cells;
• incubating said transfected eucaryotic cells;
• and
• selecting from said transfected eucaryotic cells at least one first cell line exhibiting high yield of the stress protein expressed as a result of said transfection.

A functional recombinant product produced by the steps of:

• introducing a genetic material into a procaryotic cell line coding for expression of said recombinant product;
• inserting at least one stress protein expression vector into said procaryotic cell line so as to form a modified cell line;
• selecting from said modified cell line at least one first cell line exhibiting enhanced yield of the desired functional recombinant product;
• propagating said first cell line;
• and
• collecting functional recombinant product produced by said propagated first cell line.

A functional recombinant product produced by the steps of:

• inserting at least one stress protein expression vector into an eucaryotic cell line to make a modified cell line.
• selecting from said modified cell line at least one first cell line exhibiting enhanced yield of the desired functional recombinant product;
• propagating said first cell line;
• and
• collecting functional recombinant product produced by said propagated first cell line.