Protein expression system arrays and use in biological screening

A method for screening a plurality of proteins for their ability to interact with a component of a sample comprising the steps of:

• (a) generating a protein expression array, wherein the array comprises:
• (i) a substrate;
• (ii) a binding surface which covers some or all of the substrate surface;
• and
• (iii) a plurality of protein expression systems located at discrete positions on portions of the substrate covered by the binding surface;
• and
• (b) detecting either directly or indirectly the interaction of the component with proteins expressed at specific positions comprising the protein expression systems.

A method for the detection of chemical or biological components immobilized on a solid phase by multidimensional spectroscopy (MDS) utilizing ion mobility and time of flight mass spectroscopy comprising the steps of:

• (a) recovering at least a portion of a chemical or biological mixture immobilized on a solid substrate as an electrospray;
• (b) directing the electrospray to an ion mobility chamber which separates the constituents of the directed electrospray by size, ionic charge, and shape;
• and
• (c) analyzing the separated constituents which emerge from the ion chamber by time-of-flight spectroscopy.