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US 2001/0041334 A1Published application
Stable compositions for nucleic acid amplification and sequencing
OwnerAyoub Rashtchian, Joseph Solusinventors
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OwnerAyoub Rashtchian, Joseph Solusinventors
Estimating the term…
The present invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stablizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperatures above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction) and sequencing (e.g., by dideoxy or “Sanger” sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.
A stable composition comprising a mixture of reagents at working concentrations, wherein said reagents are at least one thermostable enzyme and at least one buffer salt.
A stable composition for nucleic acid amplification comprising a mixture of reagents, wherein said reagents are at least one thermostable DNA polymerase, at least one buffer salt and at least one deoxynucleoside triphosphate.
A stable composition for nucleic acid sequencing comprising a mixture of reagents, wherein said reagents are at least one thermostable DNA polymerase, at least one deoxynucleoside triphosphate, at least one dideoxynucleoside triphosphate and at least one buffer salt.
A nucleic acid amplification kit comprising one or more containers, wherein a first container contains a stable composition comprising a mixture of reagents, wherein said reagents are at least one thermostable DNA polymerase, at least one buffer salt, and at least one deoxynucleoside triphosphate.
A nucleic acid sequencing kit comprising one or more containers, wherein a first container contains a stable composition comprising a mixture of reagents, wherein said reagents are at least one thermostable DNA polymerase, at least one buffer salt, at least one deoxynucleoside triphosphate and at least one dideoxynucleoside triphosphate.
A method of amplifying a nucleic acid molecule comprising contacting said nucleic acid molecule with a composition selected from the group consisting of:
A method of sequencing a nucleic acid molecule comprising contacting said nucleic acid molecule with a composition selected from the group consisting of:
Dependencies are inferred from the claim text.