DNA molecules encoding cartilage-derived morphogenetic proteins
OwnerFrank P. Luyten, Malcolm Moos JR. +1inventors
InventorsFrank P. Luyten, Malcolm Moos JR., Steven Chao-Huan Chang
Life of this patent
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Abstract
Nucleotide and amino acid sequences of cartilage-derived morphogenetic proteins (CDMP-1 and CDMP-2) from human and bovine cartilage extracts. These proteins exhibit chondrogenic activity and can be used to repair cartilage defects in a mammal.
Claims26
A purified cartilage extract that stimulates local cartilage formation when combined with a matrix and implanted into a mammal, said extract being produced by a method comprising:
- (a) obtaining cartilage tissue;
- (b) homogenizing said cartilage tissue in the presence of chaotropic agents under conditions that permit separation of proteins from proteoglycans;
- (c) separating said proteins from said proteoglycans;
- and
- (d) obtaining said proteins.
A method of preparing a partially purified articular cartilage extract having chondrogenic activity, comprising the steps:
- (a) obtaining cartilage tissue;
- (b) homogenizing said cartilage tissue in the presence of chaotropic agents under conditions that permit separation of proteins from proteoglycans;
- (c) separating said proteins from said proteoglycans;
- and
- (d) obtaining said proteins.
An isolated DNA molecule encoding a protein having chondrogenic activity in vivo but substantially no osteogenic activity in vivo, said molecule having a nucleotide sequence that can hybridize to a polynucleotide having a nucleotide sequence selected from the group consisting of SEQ ID NO:11 and SEQ ID NO:12 at 55° C. with 0.4×SSC and 0.1% SDS.
A recombinant protein having chondrogenic activity in vivo but substantially no osteogenic activity in vivo, wherein said protein has an amino acid sequence selected from the group consisting of SEQ ID NO:13 and SEQ ID NO:14.
A method of stimulating cartilage formation in a mammal, comprising the steps:
- a) supplying cartilage-derived morphogenetic proteins having in vivo chondrogenic activity;
- b) mixing said partially purified proteins with a matrix to produce a product that facilitates administration of said partially purified proteins;
- and
- c) implanting the product of step (b) into the body of mammal to stimulate cartilage formation at the site of implantation.
A composition that can be administered to a mammal for the purpose of stimulating chondrogenic activity at the site of administration without substantially stimulating osteogenic activity, said composition comprising at least one cartilage-derived morphogenetic protein and a matrix.
Dependencies are inferred from the claim text.