US 2001/0037014 A1 · Thin mineralized collagen membrane and method of making same

Abstract

A strong, thin flexible mineralized collagen membrane is disclosed that is useful for such medical applications as a barrier for guided tissue regeneration, periodontal defect repair, bone grafts and skin wound repair. The mineralized collagen membrane comprises a substantially homogeneous mineralized collagen composite consisting essentially of about 30% to about 70% by weight of a collagen component and about 30% to about 70% by weight of a calcium phosphate minerals component precipitated from a collagen slurry by a soluble calcium ion-containing solution and a soluble phosphate ion-containing solution. The calcium phosphate minerals component has a mole ratio of calcium to phosphate in the range of about 1.0 to about 2.0. The thin mineralized collagen membrane has a bulk density of the membrane of at least about 1.0 g/cm3 and has a thickness not greater than about 0.5 mm and preferably not greater than about 0.3 mm. A corresponding method of making the mineralized collagen membrane includes casting washed mineralized collagen slurry as a thin, flat sheet onto a surface, such as a sieve or filter paper, air drying the sheet and removing it from the surface as the thin mineralized collagen membrane.

Claims33

Dependent claims

A thin mineralized collagen membrane useful for medical applications comprises a substantially homogeneous mineralized collagen composite consisting essentially of about 30% to about 70% by weight of a collagen component and about 30% to about 70% by weight of a calcium phosphate minerals component precipitated from a collagen slurry by a soluble calcium ion-containing solution and a soluble phosphate ion-containing solution, said thin mineralized collagen membrane having a thickness not greater than about 0.5 mm.

A method for producing a substantially homogeneous thin mineralized collagen membrane comprising the steps of:

a. forming a collagen slurry;
b. forming a soluble calcium ion-containing solution;
c. forming a soluble phosphate ion containing solution;
d. adding said soluble calcium ion-containing solution and said soluble phosphate ion-containing solution to said collagen slurry while stirring the collagen slurry and maintaining the pH of said collagen slurry at least about 7, thereby inducing the precipitation of calcium phosphate mineral in the collagen slurry as mineralized collagen slurry;
e. recovering said mineralized collagen slurry by solid-liquid separation;
f. washing the recovered mineralized collagen slurry with pure water;
g. casting the washed mineralized collagen slurry into a thin sheet;
and
h. drying said thin sheet to form a thin mineralized collagen membrane having a thickness not greater than about 0.5 mm.

6 more elements

A method for producing a substantially homogeneous thin mineralized collagen membrane comprising the steps of:

a. forming a collagen slurry;
b. forming a soluble calcium ion-containing solution;
c. forming a soluble phosphate ion containing solution;
d. adding said soluble calcium ion-containing solution and said soluble phosphate ion-containing solution to said collagen slurry while stirring the collagen slurry and maintaining the pH of said collagen slurry in the range of at least about 7, thereby inducing the precipitation of calcium phosphate mineral in the collagen slurry as mineralized collagen slurry, said soluble phosphate ion containing solution being formed so that the mineralized collagen membrane comprises about 10% to about 70% calcium phosphate minerals and about 30% to about 70% collagen;
e. aging the collagen slurry for sufficiently long after the step of adding said soluble calcium ion-containing solution and said soluble phosphate ion-containing solution to said collagen to ensure at least substantially complete precipitation of calcium phosphate;
f. recovering said mineralized collagen slurry by solid-liquid separation;
g. washing the recovered mineralized collagen slurry with pure water;
h. casting the washed mineralized collagen slurry into a thin flat sheet on a filter media;
and
i. drying said thin flat sheet and removing it from the filter media as a thin mineralized collagen membrane having a thickness not greater than about 0.3 mm.

7 more elements

Dependencies are inferred from the claim text.

Thin mineralized collagen membrane and method of making same

Life of this patent

Thin mineralized collagen membrane and method of making same

Life of this patent

Abstract

Claims33