Method of preparing cryogenically preserved adherent cell containing plate for tissue culture applications

Abstract

A process for applying and storing adherent cells on a tissue culture plate 20 for preparing tissue culture plates for use in tissue culture experiments. In the process, adherent cells are removed from a flask using trypsin after being incubated in a CO2 Incubator. The cell suspension is created using the adherent cells and a liquid culture medium, which is centrifuged and tested for volume using a hemacytometer. The cell suspension is placed in the wells of a tissue culture plate 20 and then incubated. A cryopreservative is deposited into each well and the tissue culture plate is sealed with an adhesive foil cover and cryogenically preserved using liquid nitrogen. The tissue culture plate 20 is then place into a plastic, waterproof, resealable bag enabling it to be stored at −80° C. for up to 3 months and shipped without damaging the morphological or functional nature of the adherent cells. The tissue culture plate forms part of a cryopreserved cell culture assembly which includes frozen adherent cells attached to the bottom wall surface of a well in the tissue culture plate.

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