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US 2001/0036634 A1Published application
Lateral flow PCR with amplicon concentration and detection
OwnerTimothy Chow, Timothy Allan Browninventors
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Abstract
The present invention provides an apparatus and method for performing nucleic acid amplification (PCR), concentration and detection on a porous membrane. The apparatus of the present invention is of low cost and is amenable to use in the clinic for rapid and facile analysis of patient samples.
Claims28
An apparatus for thermocycling a reaction mixture in a continuous flow comprising:
- a lateral flow device having proximal and distal ends, wherein said lateral flow device comprises
- (a) a sample reservoir located at said proximal end of said lateral flow device;
- (b) a wicking pad located at said distal end of said lateral flow device;
- and
- (c) a porous membrane located between and contacting said sample reservoir and said wicking pad;
- and
- an instrument for receiving said lateral flow device comprising a temperature block comprising a plurality of stationary thermal zones, said temperature block arranged to fit between said sample reservoir and said wicking pad and in contact with said porous membrane.
4 more elements
A method for amplifying nucleic acid comprising,
- applying a nucleic acid amplification reaction mixture to the proximal end of a porous membrane;
- and
- allowing said reaction mixture to travel toward the distal end of said porous membrane, wherein said reaction mixture travels through a plurality of proximal stationary thermal zones in contact with said porous membrane.
Dependencies are inferred from the claim text.