Mixed cell diagnostic systems
AssigneeDiagnostic Hybrids, Inc.
InventorsDavid R. Scholl, Yung T. Huang, Patricia Gail Ray Goodrum
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Abstract
The present invention generally relates to the field of diagnostic microbiology, and, more particularly, to compositions and methods for detecting and differentiating one or more viruses or other intracellular parasites present in a specimen. The present invention also provides compositions and methods to evaluate the susceptibility of a organisms to antimicrobial agents.
Claims9
A method for the detection of enterovirus in a sample, comprising:
- a) providing:
- i) a sample suspected of containing enterovirus;
- and
- ii) a mixed cell culture comprising at least two cell types, wherein said cell types comprise RD cells and human mucoepidermoid cells;
- b) inoculating said mixed cell culture with at least a portion of said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of said enterovirus.
A method for the detection of enterovirus in a sample, comprising:
- a) providing:
- i) a sample suspected of containing enterovirus;
- and
- ii) a mixed cell culture comprising at least two cell types, wherein one of said cell types comprises Caco-2 cells;
- b) inoculating said mixed cell culture with at least a portion of said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of said enterovirus.
A method for the detection and identification of parainfluenza virus in a sample, comprising the steps of:
- a) providing:
- i) a sample suspected of containing parainfluenza virus;
- and
- ii) a mixed cell culture comprising at least two cell types, wherein one of said cell types comprises mink lung cells;
- b) inoculating said mixed cell culture with at least a portion of said sample to produce an inoculated culture;
- and
- c) observing said inoculated culture for the presence of said parainfluenza virus.
Dependencies are inferred from the claim text.