Cell separation method
AssigneeAsahi Medical Co., Ltd.
InventorsMasaya Sumita, Shuji Terashima
Life of this patent
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Abstract
A cell separation method comprising steps of introducing a cell-containing fluid containing cells to be recovered and cells to be removed, into a cell-capturing means capable of substantially capturing the cells to be recovered and substantially permitting passage therethrough of cells to be removed; taking out the resulting fluid containing the cells to be removed, from the cell-capturing means; and then introducing a liquid with a viscosity of not more than 500 mPa·s and not less than 5 mPa·s into the cell-capturing means to recover therefrom the cells to be recovered which have been captured by the cell-capturing means.
Claims11
A liquid containing hematopoietic stem cells which is substantially free from erythrocytes and platelets, and which has a viscosity of not more than 500 mPa·s and not less than 5 mPa·s.
A liquid containing cells to be recovered and substantially no cells to be removed which is obtained by a cell separation method comprising steps of introducing a cell-containing fluid containing cells to be recovered and cells to be removed into a cell-capturing means capable of substantially capturing said cells to be recovered and substantially permitting passage therethrough of said cells to be removed;
- taking out the resulting fluid containing the cells to be removed from said cell-capturing means;
- and then introducing a liquid with a viscosity of not more than 500 mPa·s and not less than 5 mPa·s into said cell-capturing means to recover therefrom said cells to be recovered which have been captured by said cell-capturing means.
A liquid for recovering captured cells from a cell-capturing means which has a viscosity of not more than 500 mpa·s and not less than 5 mPa·s.
Dependencies are inferred from the claim text.