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AssigneeCeltrix Pharmaceuticals, Inc.
InventorsEmerald Martin Spencer, Carol Talkington-Verser
Estimating the term…
This invention relates to polypeptides that are human somatomedin carrier protein subunits and to processes for producing them. The carrier protein subunits bind to human somatomedin-like polypeptides, also known as insulin-like growth factors. The process involves preparation from a human serum fraction, Cohn IV-1, by a molecule of various chromatographic steps. This invention also relates to DNA molecules encoding human somatomedin carrier protein-like polypeptides, recombinant DNA molecules, hosts, processes for producing carrier protein-like polypeptides, human somatomedin carrier protein-like polypeptides produced using those molecules, hosts and processes. The invention relates to DNA molecules and their expression in appropriate hosts. The recombinant DNA molecules contain DNA molecules that code for polypeptides which have a biological activity of the human carrier protein or a human carrier protein subunit capable of binding somatomedins. The DNA molecules, recombinant DNA molecules, hosts, and processes of this invention may be used in the production of polypeptides useful in a variety of therapeutic, diagnostic, and other useful applications.
A carrier protein subunit capable of binding somatomedin-like polypeptides.
A carrier protein subunit capable of binding somatomedins characterized by an N-terminal amino acid sequence of the formula:
An essentially pure carrier protein subunit capable of binding somatomedin-like polypeptides.
A process for producing a carrier protein subunit from human plasma Cohn fraction IV-1 comprising:
A DNA molecule comprising a gene which codes for a carrier protein-like polypeptide.
A DNA molecule comprising a gene which codes for a carrier protein-like polypeptide that is a, carrier protein subunit capable of binding somatomedin-like polypeptides.
A process for obtaining a DNA molecule, comprising preparing cDNA molecules from mRNA found in cells or tissues that produce the carrier protein, determining which of the cDNA molecules hybridize to one or more labelled polynucleotide probes based on the DNA sequence of FIG. 4, analyzing the cDNA molecules that hybridized, and obtaining a DNA molecule having a gene which codes for a carrier protein-like polypeptide.
A DNA molecule selected from the group consisting of the DNA molecules LCP 0.70, LCP 0.77, LCP 2.3, LCP 2.5, FCP 1.8, and FCP 2.5, DNA molecules which hybridize to any of the DNA molecules LCP 0.70, LCP 0.77, LCP 2.3, LCP 2.5, FCP 1.8 and FCP 2.5 and which code for a carrier protein-like polypeptide, and DNA molecules which code for a polypeptide coded for by any of the foregoing DNA molecules.
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids −1 to 290 of FIG. 4.
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 1 to 290 of FIG. 4.
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 27 to 290 of FIG. 4.
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 27 to 290 of FIG. 4 and having a methionine residue preceding amino acid 27.
A DNA molecule comprising a gene which codes for a polypeptide having the sequence of amino acids 27 to 290 and having a sequence of amino acid residues preceding amino acid 27 that constitute a secretion, signal or other precursor sequence recognized by a host.
An essentially pure carrier protein-like polypeptide other than a carrier protein subunit capable of binding somatomedin-like polypeptides.
A carrier protein-like polypeptide lacking the natural glycosylation of the carrier protein.
An essentially pure polypeptide having the sequence of amino acids −1 to 290 of FIG. 4.
An essentially pure polypeptide having the sequence of amino acids 1 to 290 of FIG. 4.
An essentially pure polypeptide having the sequence of amino acids 27 to 290 of FIG. 4 and such a polypeptide having a methionine residue preceding amino acid 27.
A polypeptide selected from the group consisting of a polypeptide having the sequence of amino acids −1 to 290 of FIG. 4, and polypeptides having a portion of that sequence and constituting a carrier protein-like polypeptide.
An oligonucleotide probe having all or a portion of the DNA sequence of any one of the DNA molecules LCP 2.3, LCPO 0.70, LCP 0.77, LCP 2.5, FCP 1.8 and FCP 2.5, which selectively hybridizes to a DNA molecule encoding a carrier protein-like polypeptide.
Dependencies are inferred from the claim text.